Kinematic Changes in a Mouse Model of Penetrating Hippocampal Injury and Their Recovery After Intranasal Administration of Endometrial Mesenchymal Stem Cell-Derived Extracellular Vesicles
| Autor: | Lilia Carolina León-Moreno, Rolando Castañeda-Arellano, Irene Guadalupe Aguilar-García, María Fernanda Desentis-Desentis, Elizabeth Torres-Anguiano, Coral Estefanía Gutiérrez-Almeida, Luis Jesús Najar-Acosta, Gerardo Mendizabal-Ruiz, César Rodolfo Ascencio-Piña, Judith Marcela Dueñas-Jiménez, Jorge David Rivas-Carrillo, Sergio Horacio Dueñas-Jiménez |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Angiogenesis kinematic analysis Hippocampus Inflammation Biology Hippocampal formation Neuroprotection lcsh:RC321-571 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound penetrating hippocampal injury 0302 clinical medicine endometrial mesenchymal stem cells medicine lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Original Research Mesenchymal stem cell Vascular endothelial growth factor neuronal preservation 030104 developmental biology chemistry Cellular Neuroscience medicine.symptom extracellular vesicles 030217 neurology & neurosurgery Pyknosis |
| Zdroj: | Frontiers in Cellular Neuroscience, Vol 14 (2020) Frontiers in Cellular Neuroscience |
| ISSN: | 1662-5102 |
| Popis: | Locomotion speed changes appear following hippocampal injury. We used a hippocampal penetrating brain injury mouse model to analyze other kinematic changes. We found a significant decrease in locomotion speed in both open-field and tunnel walk tests. We described a new quantitative method that allows us to analyze and compare the displacement curves between mice steps. In the tunnel walk, we marked mice with indelible ink on the knee, ankle, and metatarsus of the left and right hindlimbs to evaluate both in every step. Animals with hippocampal damage exhibit slower locomotion speed in both hindlimbs. In contrast, in the cortical injured group, we observed significant speed decrease only in the right hindlimb. We found changes in the displacement patterns after hippocampal injury. Mesenchymal stem cell-derived extracellular vesicles had been used for the treatment of several diseases in animal models. Here, we evaluated the effects of intranasal administration of endometrial mesenchymal stem cell-derived extracellular vesicles on the outcome after the hippocampal injury. We report the presence of vascular endothelial growth factor, granulocyte-macrophage colony-stimulating factor, and interleukin 6 in these vesicles. We observed locomotion speed and displacement pattern preservation in mice after vesicle treatment. These mice had lower pyknotic cells percentage and a smaller damaged area in comparison with the nontreated group, probably due to angiogenesis, wound repair, and inflammation decrease. Our results build up on the evidence of the hippocampal role in walk control and suggest that the extracellular vesicles could confer neuroprotection to the damaged hippocampus. |
| Databáze: | OpenAIRE |
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