Injectable, photoresponsive hydrogels for delivering neuroprotective proteins enabled by metal-directed protein assembly
| Autor: | Xiaotian Liu, Fei Sun, Kai Liu, Jiren Luo, Zhongguang Yang, Songzi Kou, Chao Yang, Bojing Jiang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cell signaling
genetic structures Materials Science Biocompatible Materials Protein chemistry complex mixtures Neuroprotection Mice medicine Animals Axon Research Articles chemistry.chemical_classification Multidisciplinary Biomolecule technology industry and agriculture Proteins SciAdv r-articles Photoreceptor protein Hydrogels eye diseases Axons Transition metal ions Nerve Regeneration medicine.anatomical_structure Applied Sciences and Engineering chemistry Self-healing hydrogels Biophysics sense organs Research Article |
| Zdroj: | Science Advances |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.abc4824 |
| Popis: | Injectable, photoresponsive protein hydrogels engender neuroprotection and neuroregeneration in optic nerves. Axon regeneration constitutes a fundamental challenge for regenerative neurobiology, which necessitates the use of tailor-made biomaterials for controllable delivery of cells and biomolecules. An increasingly popular approach for creating these materials is to directly assemble engineered proteins into high-order structures, a process that often relies on sophisticated protein chemistry. Here, we present a simple approach for creating injectable, photoresponsive hydrogels via metal-directed assembly of His6-tagged proteins. The B12-dependent photoreceptor protein CarHC can complex with transition metal ions through an amino-terminal His6-tag, which can further undergo a sol-gel transition upon addition of AdoB12, leading to the formation of hydrogels with marked injectability and photodegradability. The inducible phase transitions further enabled facile encapsulation and release of cells and proteins. Injecting the Zn2+-coordinated gels decorated with leukemia inhibitory factor into injured mouse optic nerves led to prolonged cellular signaling and enhanced axon regeneration. This study illustrates a powerful strategy for designing injectable biomaterials. |
| Databáze: | OpenAIRE |
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