Biosynthesis of pyocyanin pigment by Pseudomonas aeruginosa
| Autor: | M. Z. El-Fouly, Azza Adel-Fattah Mohamed Shahin, Adel M. Sharaf, Ahmed M. A. Omara, Heba A. El-Bialy |
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| Rok vydání: | 2015 |
| Předmět: |
Antimicrobial activity
medicine.disease_cause Nuclear magnetic resonance Microbiology chemistry.chemical_compound Pigment Pyocyanin Biosynthesis medicine lcsh:Nuclear and particle physics. Atomic energy. Radioactivity 16S rRNA Mass spectrometry biology Cotton seed meal Pseudomonas aeruginosa Antimicrobial biology.organism_classification 16S ribosomal RNA Yeast chemistry visual_art visual_art.visual_art_medium lcsh:QC770-798 Bacteria |
| Zdroj: | Journal of Radiation Research and Applied Sciences, Vol 8, Iss 1, Pp 36-48 (2015) |
| ISSN: | 1687-8507 |
| Popis: | Sixty-three isolates belonging to the genus Pseudomonas were isolated from different environmental sources including; soil, water and clinical specimens. Twenty out of them were identified as Pseudomonas aeruginosa and individually screened for pyocyanin production. P. aeruginosa R 1; isolated from rice-cultivated soil and P. aeruginosa U 3 selected from clinical specimen (Urinary tract infection) were the highest pyocyanin producers; pyocyanin production reached 9.3 and 5.9 μg/ml, respectively on synthetic glucose supplemented nutrient medium (GSNB). The identification of both selected strains ( P. aeruginosa R 1 and P. aeruginosa U 3 ) was confirmed by 16S rRNA, the similarity with other strains available in database was 97% (with P. aeruginosa FPVC 14) and 94% (with P. aeruginosa 13.A), respectively. P. aeruginosa R 1 and P. aeruginosa U 3 are accessed at gene bank with accession numbers KM924432 and KM603511, in the same order. Pyocyanin was extracted by standard methods, purified by column chromatography and characterized by UV-Vis absorption, mass spectrometry and nuclear magnetic resonance. The antimicrobial activity of purified pyocyanin against multi-drug resistant microbes was investigated; the efficiency of pyocyanin was more obvious in Gram +ve bacteria than Gram−ve bacteria and yeast. To reduce the cost of pyocyanin production, a new conventional medium based on cotton seed meal supplemented with peptone was designed. The pyocyanin production of both selected strains P. aeruginosa R 1 and P. aeruginosa U 3 using the new medium is increased by 30.1% and 17.2%, respectively in comparison with synthetic GSNB medium, while the cost of production process is reduced by 56.7%. |
| Databáze: | OpenAIRE |
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