Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures
| Autor: | Ana Celia Muñoz-Muñoz, Jesús Cervantes-Martínez, Benjamín Rodríguez-Garay, J.M. Rodríguez-Domínguez, Antonia Gutiérrez-Mora, Humberto Gutiérrez-Pulido |
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| Rok vydání: | 2007 |
| Předmět: |
endocrine system
Organogenesis Materials Science (miscellaneous) Cefotaxime Industrial and Manufacturing Engineering Fluorescence spectroscopy Magnoliopsida Tissue culture Optics Business and International Management Laser-induced fluorescence reproductive and urinary physiology Plant Proteins Chemistry business.industry Lasers Fluorescence In vitro Plant Leaves Spectrometry Fluorescence embryonic structures Shoot business hormones hormone substitutes and hormone antagonists Explant culture |
| Zdroj: | Applied Optics. 46:2138 |
| ISSN: | 1539-4522 0003-6935 |
| Popis: | We demonstrate the effectiveness of laser-induced fluorescence (LIF) for monitoring the development and stress detection of in vitro tissue cultures in a nondestructive and noninvasive way. The changes in LIF spectra caused by the induction of organogenesis, the increase of the F690/F740 ratio as a result of the stress originated in the organogenic explants due to shoot emergence, and the relationship between fluorescence spectra and shoot development were detected by LIF through closed containers of Saintpaulia ionantha. |
| Databáze: | OpenAIRE |
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