Differences in adipocyte long chain fatty acid uptake in Osborne–Mendel and S5B/Pl rats in response to high-fat diets
| Autor: | Paul D. Berk, Michael W. Bradbury, A F Cheema, O Petrescu, X. Fan |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Medicine (miscellaneous) Adipokine Adipose tissue Weight Gain Mitochondrial Proteins chemistry.chemical_compound Insulin resistance Species Specificity Lectins Internal medicine Adipocyte Coenzyme A Ligases Adipocytes medicine Animals Insulin chemistry.chemical_classification Nutrition and Dietetics Leptin digestive oral and skin physiology Fatty acid medicine.disease Dietary Fats Rats Endocrinology Adipose Tissue chemistry medicine.symptom Weight gain hormones hormone substitutes and hormone antagonists |
| Zdroj: | International Journal of Obesity. 32:853-862 |
| ISSN: | 1476-5497 0307-0565 |
| DOI: | 10.1038/sj.ijo.0803792 |
| Popis: | To determine whether strain differences in adipocyte uptake of long chain fatty acids (LCFAs) contribute to differences in weight gain by Osborne-Mendel (OM) and S5B/Pl rats (S) fed a high-fat diet (HFD).Ninety-four adult (12-14-week old) male OM and S rats.Body weight; epididymal fat pad weight; adipocyte size, number, LCFA uptake kinetics; and plasma insulin and leptin during administration of HFD or chow diets (CDs).In both strains, rate of weight gain (RWG) was greater on an HFD than a CD; RWG on an HFD was greater, overall, in OM than S. A significant RWG increase occurred on days 1 and 2 in both strains. It was normalized in S by days 6-9 but persisted at least till day 14 in OM. RWGs were significantly correlated (P0.001) with the V(max) for saturable adipocyte LCFA uptake (V(max)). In S, an increase in V(max) on day 1 returned to baseline by day 7 and was correlated with both plasma insulin and leptin levels throughout. In OM, a greater increase in V(max) was evident by day 2, and persisted for at least 14 days, during which both insulin and leptin levels remained elevated. Growth in epididymal fat pads on the HFD correlated with body weight, reflecting hypertrophy in OM and both hypertrophy and hyperplasia in S.(a) Changes in V(max) contribute significantly to changes in RWG on HFDs. (b) There are important strain differences in circulating insulin and leptin responses to an HFD. (c) Both insulin and leptin responses to an HFD are closely correlated with V(max) of adipocyte fatty acid uptake in S animals, but suggest early onset of insulin resistance in OM. Thus, differences in hormonal regulation of adipocyte LCFA uptake may underlie the different responses of OM and S to HFD. |
| Databáze: | OpenAIRE |
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