LncRNA00492 is required for marginal zone B-cell development
| Autor: | Liling Chen, Faming Wang, Demin Wang, Yingying Shao, Liudi Yuan, Qingyun Zhang, Baijiao Zheng, Yao Luo, Dongya Cui |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Immunology
Spleen Models Biological Immunophenotyping CTBP1 Mice Ubiquitin Bone Marrow Marginal zone B-cell medicine Immunology and Allergy Animals Receptor Notch2 Mice Knockout B-Lymphocytes biology Lymphopoiesis Ubiquitination Cell Differentiation medicine.disease Marginal zone Lymphoma Cell biology DNA-Binding Proteins Alcohol Oxidoreductases medicine.anatomical_structure Gene Expression Regulation biology.protein RNA Long Noncoding Bone marrow Nucleus Biomarkers Protein Binding Signal Transduction |
| Zdroj: | ImmunologyREFERENCES. 165(1) |
| ISSN: | 1365-2567 |
| Popis: | B-cell development undergoes a series of steps from the bone marrow to the secondary lymphoid organs. A defect in B-cell development can lead to immunodeficiency or malignant disorders, such as leukaemia or lymphoma. Long non-coding RNAs have been reported to act as important regulators of many pathological processes. However, very little is known regarding the role of lncRNAs during B-cell development and the regulation of their expression. In this study, we explored the expression and role of lncRNA Gme00492 in B-cell development. We observed that lnc00492 was highly expressed in B-cell development and primarily expressed in the nucleus. Lnc00492-deficient mice had fewer marginal zone B cells in the spleen, likely due to a developmental block. Importantly, lnc00492 interacts with CTBP1 and targets it for ubiquitination and degradation during B-cell development, whereas the transcriptional corepressor factor CTBP1 plays a critical role in Notch2 signalling. Thus, we identified a novel regulatory axis between lnc00492 and CTBP1 in B cells, suggesting that lnc00492 is essential for marginal zone B-cell development. |
| Databáze: | OpenAIRE |
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