Evaluation of halothane sensitivity and prediction of post-mortem muscle metabolism in pigs from a muscle biopsy using 31P NMR spectroscopy
| Autor: | R. Lahucky, J. Mojto, Jean-Pierre Renou, A. Miri, A. Talmant, Gabriel Monin, J. Poltarsky |
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| Přispěvatelé: | ProdInra, Migration, Station de recherches sur la viande, Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 1993 |
| Předmět: |
PHOSPHORE 31
Belgian Landrace Creatine 03 medical and health sciences chemistry.chemical_compound Animal science [SDV.IDA]Life Sciences [q-bio]/Food engineering Biopsy medicine ComputingMilieux_MISCELLANEOUS 030304 developmental biology 2. Zero hunger 0303 health sciences Muscle biopsy biology medicine.diagnostic_test Chemistry 0402 animal and dairy science 04 agricultural and veterinary sciences Metabolism [SDV.IDA] Life Sciences [q-bio]/Food engineering biology.organism_classification Phosphate 040201 dairy & animal science Biceps femoris muscle Biochemistry Halothane Food Science medicine.drug |
| Zdroj: | Meat Science Meat Science, Elsevier, 1993, 33 (3), pp.373-384 |
| ISSN: | 0309-1740 |
| DOI: | 10.1016/0309-1740(93)90008-6 |
| Popis: | Phosphorus nuclear magnetic resonance ((31)P NMR) measurements were made on biopsy samples from pig muscles. Two experiments were performed, one in France and one in Czechoslovakia. In experiment 1, the muscle samples were obtained by surgery from the Biceps femoris muscle of 10 pigs with various genetic types (5 Large White, 1 Large White × Landrace, 4 Pietrains). In experiment 2, the muscle samples were obtained by shot biopsy from the Longissimus dorsi muscle of 11 Belgian Landrace × Duroc pigs, of which 6 were halothane-negative and 5 halothane-positive. The pigs were slaughtered by electrostunning and exsanguination respectively 3 weeks and 1 week after the biopsy, and meat quality traits (pH(1), reflectance and water holding capacity) were determined. The changes in adenosine triphosphate (ATP), creatine phosphate (CP), inorganic phosphate (P(i)) and pH, as deduced from NMR, were faster in Pietrains than in Large Whites, and in halothane-positive than in halothane-negative pigs. The value of the CP/P(i) ratio at 40 min after taking biopsy allowed discrimination between halothane-positive and halothane-negative pigs. The values of pH, creatine phosphate and adenosine triphosphate, as measured at definite times on the biopsies, were correlated with the rate of post-mortem metabolism (r approx 0·8) and with meat quality traits. (31)P NMR measurements combined with a rapid and efficient biopsy technique appear as an alternative tool for assessment of both halothane sensitivity and prediction of meat quality in pigs. |
| Databáze: | OpenAIRE |
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