Two-dimensional liquid chromatographic analysis of ramelteon in human serum
| Autor: | Sıdıka Ertürk Toker, Armağan Önal, Olcay Sagirli, S. Evrim Kepekci Tekkeli |
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| Přispěvatelé: | TEKKELİ, ŞERİFE EVRİM |
| Rok vydání: | 2015 |
| Předmět: |
Accuracy and precision
Chromatography Sagirli O. Onal A. Toker S. E. TEKKELİ Ş. E. -Two-dimensional liquid chromatographic analysis of ramelteon in human serum- ARABIAN JOURNAL OF CHEMISTRY cilt.12 ss.2817-2822 2019 Chemistry(all) Calibration curve General Chemical Engineering Ramelteon Extraction (chemistry) Analytical chemistry General Chemistry Serum samples lcsh:Chemistry chemistry.chemical_compound chemistry lcsh:QD1-999 medicine Chemical Engineering(all) Protein precipitation Methanol Acetonitrile medicine.drug |
| Zdroj: | Arabian Journal of Chemistry, Vol 12, Iss 8, Pp 2817-2822 (2019) |
| ISSN: | 1878-5352 |
| DOI: | 10.1016/j.arabjc.2015.06.021 |
| Popis: | In this study, a selective and sensitive new 2D-LC method was developed for the determination of ramelteon in human serum. Separations were performed using the following systems: the first dimension consisted of a C18 (50 mm x 2.1 mm) pre-separation column and the mobile phase of 45% acetonitrile: 55% 10 mM ortho-phosphoric acid solution mixture and the second dimension consisted of a pentafluorophenyl propyl (150 mm x 4.6 mm) separating column and the mobile phase of 40% methanol: 60% 2.25 mM ortho-phosphoric acid solution mixture. Two systems were connected with a 6 port valve including 200 mu L sample loop and separations were performed continuously through injections. Detection wavelength has been selected as 203 nm for both systems. Extraction of drug substance from serum samples was performed using protein precipitation method with methanol and the calibration curve was linear over a concentration range of 1-50 ng/mL. The accuracy and precision of the method were determined as 1.49-4.24% and 1.75-3.63% ranges for RSD and RME, respectively. The absolute recovery of the drug was found as 80.28%. The developed method was successfully applied for the determination of ramelteon in serum samples collected from a volunteer who had received 8 mg ramelteon orally. (C) 2015 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). İstanbul Üniversitesi |
| Databáze: | OpenAIRE |
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