Analysis of reading frame and expressional regulation of randomly selected promoter-proximal genes in Escherichia coli
| Autor: | Satoshi Yanai, Mamoru Yamada, Ali Azam Talukder |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Sigma Factor Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Open Reading Frames chemistry.chemical_compound Plasmid Bacterial Proteins RNA polymerase Gene expression Escherichia coli medicine Genomic library Amino Acid Sequence ORFS Promoter Regions Genetic Gene Gene Library Genetics Base Sequence Gene Expression Regulation Bacterial Sequence Analysis DNA Molecular biology chemistry Genes Bacterial |
| Zdroj: | The Journal of General and Applied Microbiology. 51:93-103 |
| ISSN: | 1349-8037 0022-1260 |
| DOI: | 10.2323/jgam.51.93 |
| Popis: | The expression of seventy-seven randomly cloned genes of Escherichia coli was examined following a variety of treatments including heat shock, glucose starvation, phosphate starvation, ammonium starvation or osmotic shock, with the aid of lacZ reporter gene protein fusions on multicopy plasmids. Two of 77 genes (amr and yigL) had not previously been identified as protein encoding open-reading frames (ORFs) in annotations of the E. coli genome database. Thirteen genes exhibited significant changes in expression in response to at least one of the treatments, and six of them appeared to be controlled by more than one sigma (sigma) factor of RNA polymerase. This study thus allows us not only to identify the reading frame of the genomic genes but also to support the hypothesis earlier proposed that a significant proportion of genes in E. coli are involved in adaptations to various stresses to which the organism is likely to be exposed in the environment. |
| Databáze: | OpenAIRE |
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