Systemic evaluation on ischemia and reperfusion injury of splanchnic organs in rats
| Autor: | Carlos Alberto Hussni, Nereide Freire Cerqueira, Carlos Roberto Padovani, Winston Bonetti Yoshida |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Ischemia Hemodynamics Blood Pressure Hematocrit Inferior mesenteric artery Heart Rate Mesenteric Artery Superior medicine.artery Intestine Small medicine Animals Choque Superior mesenteric artery Splanchnic Circulation Rats Wistar Aorta Intestino Delgado Ratos medicine.diagnostic_test business.industry Mesenteric Artery Inferior Shock Blood flow medicine.disease Mesenteric Arteries Rats Isquemia Intestines Disease Models Animal Blood pressure Anesthesia Reperfusion Injury Reperfusion Reperfusão Surgery business Reperfusion injury |
| Zdroj: | Acta Cirúrgica Brasileira v.24 n.4 2009 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0102-8650 |
| Popis: | Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:58:13Z No. of bitstreams: 1 S0102-86502009000400008.pdf: 169017 bytes, checksum: 0c1c23ad8de50e1ed3a804ab86272f53 (MD5) Made available in DSpace on 2013-08-22T18:58:13Z (GMT). No. of bitstreams: 1 S0102-86502009000400008.pdf: 169017 bytes, checksum: 0c1c23ad8de50e1ed3a804ab86272f53 (MD5) Previous issue date: 2009-08-01 Made available in DSpace on 2013-09-30T18:15:56Z (GMT). No. of bitstreams: 2 S0102-86502009000400008.pdf: 169017 bytes, checksum: 0c1c23ad8de50e1ed3a804ab86272f53 (MD5) S0102-86502009000400008.pdf.txt: 22250 bytes, checksum: 00d37621c517d6255f842572649525c4 (MD5) Previous issue date: 2009-08-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:39:05Z No. of bitstreams: 2 S0102-86502009000400008.pdf: 169017 bytes, checksum: 0c1c23ad8de50e1ed3a804ab86272f53 (MD5) S0102-86502009000400008.pdf.txt: 22250 bytes, checksum: 00d37621c517d6255f842572649525c4 (MD5) Made available in DSpace on 2014-05-20T13:39:05Z (GMT). No. of bitstreams: 2 S0102-86502009000400008.pdf: 169017 bytes, checksum: 0c1c23ad8de50e1ed3a804ab86272f53 (MD5) S0102-86502009000400008.pdf.txt: 22250 bytes, checksum: 00d37621c517d6255f842572649525c4 (MD5) Previous issue date: 2009-08-01 OBJETIVO: Avaliar as alterações hemodinâmicas e sistêmicas decorrentes de isquemia e reperfusão (I/R) esplâncnica em ratos. MÉTODOS: Vinte ratos foram divididos em dois grupos: grupo controle: os animais foram submetidos à cirurgia, mas não a I/R e foram tratados com solução fisiológica (5 ml/kg/h) por 150 minutos; grupo I/R: os animais foram submetidos à administração contínua de solução fisiológica e à oclusão do tronco celíaco, artéria mesentérica superior e artéria mesentérica inferior por 30 minutos, seguidos por 120 minutos de reperfusão. Avaliou-se a pressão arterial média, pressão venosa, fluxo sangüíneo na aorta e na artéria mesentérica superior, freqüência cardíaca, temperatura esofágica e hematócrito. RESULTADOS: Durante a reperfusão, no grupo I/R, houve uma diminuição progressiva da pressão arterial média, fluxo sangüíneo na aorta e artéria mesentérica superior, freqüência cardíaca e temperatura esofágica; pressão venosa e hematócrito não sofreram alteração. CONCLUSÃO: O modelo de isquemia provocado por oclusão da artéria mesentérica superior, artéria mesentérica inferior e tronco celíaco por 30 minutos seguidos por 120 minutos de reperfusão provoca alterações sistêmicas evidenciadas por hipotensão, diminuição do fluxo sangüíneo mesentérico, da freqüência cardíaca e da temperatura esofágica. PURPOSE: To evaluate hemodynamic and systemic changes during and after splanchnic ischemia and reperfusion (I/R). METHODS: Rats were divided into two groups: a) control: animals submitted to surgery, but not to I/R, treated with saline (5 ml/kg/h) for 150 min; b) group I/R: animals continuously infused with saline, and submitted to occlusion of the celiac trunk, superior mesenteric artery (SMA), and inferior mesenteric artery for 30 min, followed by a 120-min reperfusion. Mean arterial blood pressure (MABP), venous pressure, aortic and SMA blood flow, heart rate, esophageal temperature and hematocrit were evaluated. RESULTS: During reperfusion in I/R groups, there was a progressive decrease in MABP, aortic blood flow, SMA blood flow, heart rate and esophageal temperature; vein pressure and hematocrit remained unchanged during the experiment. CONCLUSION: The model of ischemia used causes systemic changes, which are evidenced by hypotension, decrease in mesenteric blood flow, heart rate and esophageal temperature. UNESP FMVZ Department of Veterinary Surgery and Anesthesiology UNESP Botucatu School of Medicine Department of Surgery and Orthopedics UNESP Institute of Biosciences Department of Biostatistics UNESP FMVZ Department of Veterinary Surgery and Anesthesiology UNESP Botucatu School of Medicine Department of Surgery and Orthopedics UNESP Institute of Biosciences Department of Biostatistics |
| Databáze: | OpenAIRE |
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