Lack of interleukin 10 expression in monocyte-derived macrophages in response to in vitro infection by HIV type 1 isolates
| Autor: | Olivier Benveniste, Alexandre Lebeaut, Philippe Fretier, Philippe Jaccard, Nathalie Dereuddre-Bosquet, Dominique Dormont, Pascal Clayette, Bruno Vaslin, Marc Martin |
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| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
medicine.medical_treatment Immunology Biology Virus Replication Monocytes Multiplicity of infection Virology Gene expression medicine Macrophage Humans Secretion RNA Messenger Cells Cultured Tumor Necrosis Factor-alpha Monocyte Macrophages virus diseases Viral Load In vitro HIV Reverse Transcriptase Interleukin-10 Interleukin 10 Infectious Diseases medicine.anatomical_structure Cytokine Gene Expression Regulation HIV-1 |
| Zdroj: | AIDS research and human retroviruses. 13(11) |
| ISSN: | 0889-2229 |
| Popis: | Interleukin 10 is a pleiotropic cytokine that is overexpressed in HIV-infected patients. Here, we investigated IL-10 expression in primary cultures of monocyte-derived macrophages (MDMs) in response to in vitro infection by HIV-1/Ba-L or two macrophage-tropic HIV-1 primary isolates. Whatever the multiplicity of infection used, and in spite of high replication levels and an increase in HIV-infected cell frequency, neither significant IL-10 secretion nor IL-10 mRNA overexpression was induced in HIV-1-infected MDMs. Moreover, identical results were obtained with HIV-1-infected 1-day monocytes. These results show that MDM infection by HIV is not sufficient by itself for inducing IL-10 synthesis. |
| Databáze: | OpenAIRE |
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