Suggestive Serological Evidence of Infection with Shrew-Borne Imjin Virus (Hantaviridae) in Humans

Autor: Li-Jun Wang, Rui Qi, Xi-feng Sun, Xue-Jie Yu, Jian-Wei Liu, Min Zhao, Xiao-Ying Lei, Ling Wang, Xiang-Rong Qin, Fachun Jiang
Rok vydání: 2019
Předmět:
Zdroj: Viruses
Volume 11
Issue 12
ISSN: 1999-4915
DOI: 10.3390/v11121128
Popis: The pathogenicity of the shrew-borne Imjin virus (MJNV) is unknown. The objective of our study was to find serological evidence of MJNV infection in humans. Partial MJNV nucleocapsid protein (NP) was cloned and expressed as an antigen for double-antigen sandwich ELISA, IgM capture ELISA, and dot blot to detect MJNV specific antibodies in hemorrhagic fever with renal syndrome (HFRS) patients&rsquo
and healthy persons&rsquo
sera from endemic areas in China. The purified recombinant NP reacted with neither the 90 healthy individuals&rsquo
sera from non-endemic areas of MJNV nor the 100 antisera to HFRS-causing virus, indicating that the MJNV NP had no cross-reaction with normal human sera and HFRS-causing viral antibodies. As determined by screening ELISA and dot blot analysis, IgG antibodies against MJNV NP were detected in sera from two of 385 healthy individuals from MJNV-endemic areas, suggesting infection with MJNV or MJNV-like thottimvirus. Based on the suggestive evidence, healthcare workers should be alert to febrile diseases occurring among individuals with exposure to shrew-infested habitats.
Databáze: OpenAIRE
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