Improved synthesis and radiolabeling of [11C]MP4A, a suitable ligand for the investigation of the cholinergic system using PET
| Autor: | Ferruccio Fazio, A. Carpinelli, E. Turolla, Mario Matarrese, Fulvio Magni, Niccolò Bosso, Sergio Todde, Angela Cattaneo, M. Galli Kienle |
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| Přispěvatelé: | Carpinelli, A, Magni, F, Cattaneo, A, Matarrese, M, Turolla, E, Todde, S, Bosso, N, Kienle, M, Fazio, F |
| Rok vydání: | 2005 |
| Předmět: |
LC-APCI/MS
C-11 Magnetic Resonance Spectroscopy Hydrochloride [11C]MP4A Positron emission tomography (PET) N-methyl-4-piperidyl acetate Acetates Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Piperidines medicine Organic chemistry Humans positron emission tomography (PET) Hydrocarbons Iodinated Chromatography High Pressure Liquid N-methyl-4-piperidylacetate Radiation Chloroform Chromatography Acetylation Nuclear magnetic resonance spectroscopy Ligand (biochemistry) chemistry Isotope Labeling Positron-Emission Tomography [C-11]MP4A Anhydrous Acetylcholinesterase Gas chromatography–mass spectrometry Acetic acid-piperidine-4-yl ester Radiopharmaceuticals Acetylcholine medicine.drug |
| Zdroj: | Applied radiation and isotopes 64 (2006): 182–186. info:cnr-pdr/source/autori:Carpinelli A. 1, Magni F. 2, Cattaneo A. 2, Matarrese M. 1, Turolla E. 1, Todde S. 1, Bosso N. 2, Galli Kienle M. 2, Fazio F. 1/titolo:Improved synthesis and radiolabeling of [(11)C]MP4A, a suitable ligand for the investigation of the cholinergic system using PET/doi:/rivista:Applied radiation and isotopes/anno:2006/pagina_da:182/pagina_a:186/intervallo_pagine:182–186/volume:64 |
| ISSN: | 0969-8043 |
| Popis: | An improved synthesis of the precursor acetic acid-piperidine-4-yl ester by acetylation of 4-hydroxypiperidine hydrochloride in anhydrous chloroform was developed. A procedure for fast evaluation and characterization of products originated by acetylation of the 4-piperidinol using LC-APCI/MS with an acetonitrile-water gradient method on a Merck Purosphere RP-18 column was also developed. The highly purified precursor allowed the production of [C-11]MP4A for PET studies of acethylcholine neurotransmission system. The tracer was produced with > 98% radiochemical purity, with yields ranging 20-60% (decay-corrected) from EOB. (c) 2005 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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