Effect of cyclic uniaxial compressive stress on human dental pulp cellsin vitro
| Autor: | Qian-Hua Gao, Wen-Ling Zou, Meiying Shao, Linyan Wang, Yi-Xuan Shu, Hui Yang, Tao Hu, Li-Yang Guo |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Cellular differentiation Dentistry Biochemistry 03 medical and health sciences 0302 clinical medicine stomatognathic system Rheumatology Humans Orthopedics and Sports Medicine Molecular Biology Cells Cultured Dental Pulp Cell Proliferation Extracellular Matrix Proteins Odontoblasts business.industry Cell growth Chemistry Mechanism (biology) Cell Differentiation Epithelial Cells Cell Biology Alkaline Phosphatase In vitro stomatognathic diseases 030104 developmental biology Compressive strength 030220 oncology & carcinogenesis Exercise Test Biophysics Odontogenesis business |
| Zdroj: | Connective Tissue Research. :1-8 |
| ISSN: | 1607-8438 0300-8207 |
| DOI: | 10.1080/03008207.2017.1367773 |
| Popis: | Teeth are exposed to various forces during functional and parafunctional movements. These processes inevitably affect the dental pulp, and the mechanism of these influences has been the subject of many previous studies using different apparatuses and obtaining different results. In this study, we aimed to investigate the effects of compressive stress on the proliferation and differentiation of human dental pulp cells (hDPCs).A four-point bending strain system was adopted to apply low-density cyclic uniaxial compressive stress (2000 microstrain, 0.5 Hz) to hDPCs for 1.5, 3, 6, 12, and 24 h. The cell cycle progression and mRNA expression of differentiation-related genes (BMP2, ALP, DMP1, DSPP, COL I) were then examined to investigate the proliferation and differentiation of hDPCs.The results showed that cyclic compressive stress changed the morphology of hDPCs after 12 and 24 h of mechanical loading; cell cycle progression was promoted, especially in the 24-h group (p0.05). The expression of BMP2 was significantly upregulated after 3 and 6 h of mechanical loading but declined in the 12- and 24-h groups, whereas the expression levels of DMP1 and DSPP were significantly upregulated in the 12- and 24-h loading groups (p0.05).Dental pulp cells were sensitive to compressive stress, especially after 12 and 24 h of applied force. Proliferation and odontogenic differentiation were significantly promoted in this in vitro model. |
| Databáze: | OpenAIRE |
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