Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels
| Autor: | Alexandra H. Smith, Thomas G. Rehberger, Erik P. Lillehoj, Hyun S. Lillehoj, Noah P. Zimmerman, Inkyung Park |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
medicine.drug_class Metabolite Antibiotics Bacillus subtilis nucleoside 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Metabolomics medicine Food science 030304 developmental biology chemistry.chemical_classification 0303 health sciences lcsh:Veterinary medicine General Veterinary biology Fatty acid Fructose 04 agricultural and veterinary sciences biology.organism_classification metabolomics Amino acid probiotics chemistry gut lcsh:SF600-1100 fatty acid Nucleoside amino acid |
| Zdroj: | Frontiers in Veterinary Science, Vol 7 (2020) |
| ISSN: | 2297-1769 |
| DOI: | 10.3389/fvets.2020.00123 |
| Popis: | Direct-fed microbials (DFMs) are dietary supplements containing live microorganisms which confer a performance and health benefit to the host, but the mechanisms are unclear. Here, a metabolomics approach was used to identify changes in intestinal metabolite levels in chickens fed an unsupplemented diet or a diet supplemented with B. subtilis strain 1781 or strain 747. Body weight gains of chickens fed the B. subtilis-supplemented diets were increased up to 5.6% in the B. subtilis 1781 group and 7.6% in the B. subtilis 747 group compared with chickens fed the unsupplemented diet. Compared with unsupplemented controls, the levels of 83 metabolites were altered (p < 0.05) (25 increased, 58 decreased) in chickens given the B. subtilis 1781-supplemented diet, while 50 were altered (p < 0.05) (12 increased, 38 decreased) with the B. subtilis 747-supplemented diet. Twenty-two metabolites were altered (p < 0.05) (18 increased, 4 decreased) in the B. subtilis 1781 vs. B. subtilis 747 groups. A random forest analysis of the B. subtilis 1781 vs. control groups gave a predictive accuracy of 87.5%, while that of the B. subtilis 747 vs. control groups was 62.5%. A random forest analysis of the B. subtilis 1781 vs. B. subtilis 747 groups gave a predictive accuracy of 75.0%. Changes in the levels of these intestinal biochemicals provided a distinctive biochemical signature unique to each B. subtilis-supplemented group, and were characterized by alterations in the levels of dipeptides (alanylleucine, glutaminylleucine, phenylalanylalanine, valylglutamine), nucleosides (N1-methyladenosine, N6-methyladenosine, guanine, 2-deoxyguanosine), fatty acids (sebacate, valerylglycine, linoleoylcholine), and carbohydrates (fructose). These results provide the foundation for future studies to identify biochemicals that might be used to improve poultry growth performance in the absence of antibiotic growth promoters. |
| Databáze: | OpenAIRE |
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