The impact of ingested potato type II inhibitors on the production of the major serine proteases in the gut of Helicoverpa armigera
| Autor: | Kerry Dunse, Jackie. Stevens, Barbara L. Barbeta, Rosemary F. Guarino, S.C. Evans, Marilyn A. Anderson, Jenny West |
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| Rok vydání: | 2013 |
| Předmět: |
Proteases
Helicoverpa punctigera Molecular Sequence Data Moths Helicoverpa armigera Insect Control Biochemistry Serine medicine Animals Chymotrypsin Trypsin Amino Acid Sequence Cloning Molecular Molecular Biology Solanum tuberosum biology Plant Extracts Frass fungi biology.organism_classification Gastrointestinal Tract Polyclonal antibodies Insect Science biology.protein Insect Proteins Trypsin Inhibitors Sequence Alignment medicine.drug |
| Zdroj: | Insect Biochemistry and Molecular Biology. 43:197-208 |
| ISSN: | 0965-1748 |
| Popis: | The flowers of the ornamental tobacco produce high levels of a series of 6 kDa serine protease inhibitors (NaPIs) that are effective inhibitors of trypsins and chymotrypsins from lepidopteran species. These inhibitors have a negative impact on the growth and development of lepidopteran larvae and have a potential role in plant protection. Here we investigate the effect of NaPIs on the activity and levels of serine proteases in the gut of Helicoverpa armigera larvae and explore the adaptive mechanisms larvae employ to overcome the negative effects of NaPIs in the diet. Polyclonal antibodies were raised against a Helicoverpa punctigera trypsin that is a target for NaPIs and two H. punctigera chymotrypsins; one that is resistant and one that is susceptible to inhibition by NaPIs. The antibodies were used to optimize procedures for extraction of proteases for immunoblot analysis and to assess the effect of NaPIs on the relative levels of the proteases in the gut and frass. We discovered that consumption of NaPIs did not lead to over-production of trypsins or chymotrypsins but did result in excessive loss of proteases to the frass. |
| Databáze: | OpenAIRE |
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