A semi-automated FISH-based micronucleus-centromere assay for biomonitoring of hospital workers exposed to low doses of ionizing radiation

Autor: Veerle Decorte, Julie Depuydt, André Wambersie, Anne Vral, Hubert Thierens
Přispěvatelé: UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de radiothérapie oncologique, UCL - (SLuc) Centre du cancer
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Cancer Research
Medical surveillance
micronucleus assay
LYMPHOCYTES
Biochemistry
030218 nuclear medicine & medical imaging
Ionizing radiation
0302 clinical medicine
Radiation
Ionizing
DICENTRIC CHROMOSOMES
BIOLOGICAL DOSIMETRY
In Situ Hybridization
Fluorescence
OCCUPATIONAL DOSIMETRY
Micronucleus Tests
medicine.diagnostic_test
dosimetry
Low dose
Interventional radiology
Articles
Oncology
030220 oncology & carcinogenesis
Micronucleus test
Molecular Medicine
ionizing radiation
Environmental Monitoring
medicine.medical_specialty
Centromere
low dose exposure
Radiation Dosage
DOUBLE DOSIMETRY ALGORITHMS
03 medical and health sciences
Internal medicine
Occupational Exposure
Genetics
medicine
Dosimetry
Humans
Radiometry
Molecular Biology
business.industry
Biology and Life Sciences
INTERVENTIONAL RADIOLOGY STAFF
Dose-Response Relationship
Radiation
centromeres
Personnel
Hospital
Case-Control Studies
hospital workers
biomonitoring
DNA damage
Micronucleus
business
Nuclear medicine
Blood sampling
Zdroj: Molecular Medicine Reports
MOLECULAR MEDICINE REPORTS
Molecular Medicine Reports, Vol. 14, no.1, p. 103-110 (2016)
ISSN: 1791-3004
1791-2997
Popis: The aim of the present study was to perform cytogenetic analysis by means of a semi-automated micro-nucleus-centromere assay in lymphocytes from medical radiation workers. Two groups of workers receiving the highest occupational doses were selected: 10 nuclear medicine technicians and 10 interventional radiologists/cardiologists. Centromere-negative micronucleus (MNCM−) data, obtained from these two groups of medical radiation workers were compared with those obtained in matched controls. The blood samples of the matched controls were additionally used to construct a 'low-dose' (0–100 mGy) MNCM− dose-response curve to evaluate the sensitivity and suitability of the micronucleus-centromere assay as an 'effect' biomarker in medical surveillance programs. The physical dosimetry data of the 3 years preceding the blood sampling, based on single or double dosimetry practices, were collected for the interpretation of the micronucleus data. The in vitro radiation results showed that for small sized groups, semi-automated scoring of MNCM− enables the detection of a dose of 50 mGy. The comparison of MNCM− yields in medical radiation workers and control individuals showed enhanced MNCM− scores in the medical radiation workers group (P=0.15). The highest MNCM− scores were obtained in the interventional radiologists/cardiologists group, and these scores were significantly higher compared with those obtained from the matched control group (P=0.05). The higher MNCM− scores observed in interventional radiologists/cardiologists compared with nuclear medicine technicians were not in agreement with the personal dosimetry records in both groups, which may point to the limitation of 'double dosimetry' procedures used in interventional radiology/cardiology. In conclusion, the data obtained in the present study supports the importance of cytogenetic analysis, in addition to physical dosimetry, as a routine biomonitoring method in medical radiation workers receiving the highest occupational radiation burdens.
Databáze: OpenAIRE
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