Circulation of hepatitis delta virus and occult hepatitis B virus infection amongst HIV/HBV co-infected patients in Korle-Bu, Ghana

Autor: Yaw Awuku-Larbi, Joseph H.K. Bonney, Esinam Agbosu, John Mensah, Peter Puplampu, Seth Agyemang, Vincent Ganu, Gifty Mawuli, John Kofi Odoom, Evelyn Y. Bonney, Osbourne Quaye, Keren Attiku, James Aboagye, Augustina K. Arjarquah
Jazyk: angličtina
Rok vydání: 2021
Předmět:
RNA viruses
Male
HBsAg
Genotyping Techniques
viruses
HIV Infections
medicine.disease_cause
Pathology and Laboratory Medicine
Ghana
Geographical Locations
Immunodeficiency Viruses
Genotype
Hepatitis Viruses
Medicine and Health Sciences
Public and Occupational Health
Longitudinal Studies
Virus Testing
Multidisciplinary
biology
Transmission (medicine)
Coinfection
Incidence (epidemiology)
virus diseases
HIV diagnosis and management
Alanine Transaminase
Viral Load
Middle Aged
Hepatitis B
Vaccination and Immunization
Medical Microbiology
Viral Pathogens
Viruses
Medicine
Female
Antibody
Hepatitis Delta Virus
Pathogens
Viral load
Research Article
Adult
Hepatitis B virus
Science
Immunology
Antiretroviral Therapy
Microbiology
Virus
Young Adult
Antiviral Therapy
Diagnostic Medicine
Virology
Retroviruses
medicine
Humans
Aspartate Aminotransferases
Microbial Pathogens
business.industry
Lentivirus
Organisms
Biology and Life Sciences
HIV
digestive system diseases
People and Places
Africa
biology.protein
Preventive Medicine
business
Viral Transmission and Infection
Zdroj: PLoS ONE, Vol 16, Iss 1, p e0244507 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Background Within HIV/HBV infected patients, an increase in HDV infection has been observed; there is inadequate information on HDV prevalence as well as virologic profile in Ghana. This study sought to determine the presence of HDV in HIV/HBV co-infected patients in Ghana. Methods This was a longitudinal purposive study which enrolled 113 HIV/HBV co-infected patients attending clinic at Korle-Bu Teaching Hospital (KBTH) in Accra, Ghana. After consenting, 5 mL whole blood was collected at two-time points (baseline and 4–6 months afterwards). The sera obtained were tested to confirm the presence of HIV, HBV antibodies and/or antigens, and HBV DNA. Antibodies and viral RNA were also determined for HDV. Amplified HBV DNA and HDV RNA were sequenced and phylogenetic analysis carried out with reference sequences from the GenBank to establish the genotypes. Results Of the 113 samples tested 63 (55.7%) were females and 50 (44.25%) were males with a median age of 45 years. A total of 100 (88.5%) samples had detectable HBV surface antigen (HBsAg), and 32 out of the 113 had detectable HBV DNA. Nucleotide sequences were obtained for 15 and 2 samples of HBV and HDV, respectively. Phylogenetic analysis was predominantly genotype E for the HBVs and genotype 1 for the HDVs. Of the 13 samples that were HBsAg unreactive, 4 (30.8%) had detectable HBV DNA suggesting the incidence of occult HBV infections. The percentage occurrence of HDV in this study was observed to be 3.54. Conclusion Our data suggest the presence and circulation of HDV and incidence of occult HBV infection in HIV/HBV co-infected patients in Ghana. This informs health staff and makes it imperative to look out for the presence of HDV and occult HBV in HIV/HBV co-infected patients presenting with potential risk of liver cancers and HBV transmission through haemodialysis and blood transfusions.
Databáze: OpenAIRE
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