Circulation of hepatitis delta virus and occult hepatitis B virus infection amongst HIV/HBV co-infected patients in Korle-Bu, Ghana
Autor: | Yaw Awuku-Larbi, Joseph H.K. Bonney, Esinam Agbosu, John Mensah, Peter Puplampu, Seth Agyemang, Vincent Ganu, Gifty Mawuli, John Kofi Odoom, Evelyn Y. Bonney, Osbourne Quaye, Keren Attiku, James Aboagye, Augustina K. Arjarquah |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
RNA viruses
Male HBsAg Genotyping Techniques viruses HIV Infections medicine.disease_cause Pathology and Laboratory Medicine Ghana Geographical Locations Immunodeficiency Viruses Genotype Hepatitis Viruses Medicine and Health Sciences Public and Occupational Health Longitudinal Studies Virus Testing Multidisciplinary biology Transmission (medicine) Coinfection Incidence (epidemiology) virus diseases HIV diagnosis and management Alanine Transaminase Viral Load Middle Aged Hepatitis B Vaccination and Immunization Medical Microbiology Viral Pathogens Viruses Medicine Female Antibody Hepatitis Delta Virus Pathogens Viral load Research Article Adult Hepatitis B virus Science Immunology Antiretroviral Therapy Microbiology Virus Young Adult Antiviral Therapy Diagnostic Medicine Virology Retroviruses medicine Humans Aspartate Aminotransferases Microbial Pathogens business.industry Lentivirus Organisms Biology and Life Sciences HIV digestive system diseases People and Places Africa biology.protein Preventive Medicine business Viral Transmission and Infection |
Zdroj: | PLoS ONE, Vol 16, Iss 1, p e0244507 (2021) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Background Within HIV/HBV infected patients, an increase in HDV infection has been observed; there is inadequate information on HDV prevalence as well as virologic profile in Ghana. This study sought to determine the presence of HDV in HIV/HBV co-infected patients in Ghana. Methods This was a longitudinal purposive study which enrolled 113 HIV/HBV co-infected patients attending clinic at Korle-Bu Teaching Hospital (KBTH) in Accra, Ghana. After consenting, 5 mL whole blood was collected at two-time points (baseline and 4–6 months afterwards). The sera obtained were tested to confirm the presence of HIV, HBV antibodies and/or antigens, and HBV DNA. Antibodies and viral RNA were also determined for HDV. Amplified HBV DNA and HDV RNA were sequenced and phylogenetic analysis carried out with reference sequences from the GenBank to establish the genotypes. Results Of the 113 samples tested 63 (55.7%) were females and 50 (44.25%) were males with a median age of 45 years. A total of 100 (88.5%) samples had detectable HBV surface antigen (HBsAg), and 32 out of the 113 had detectable HBV DNA. Nucleotide sequences were obtained for 15 and 2 samples of HBV and HDV, respectively. Phylogenetic analysis was predominantly genotype E for the HBVs and genotype 1 for the HDVs. Of the 13 samples that were HBsAg unreactive, 4 (30.8%) had detectable HBV DNA suggesting the incidence of occult HBV infections. The percentage occurrence of HDV in this study was observed to be 3.54. Conclusion Our data suggest the presence and circulation of HDV and incidence of occult HBV infection in HIV/HBV co-infected patients in Ghana. This informs health staff and makes it imperative to look out for the presence of HDV and occult HBV in HIV/HBV co-infected patients presenting with potential risk of liver cancers and HBV transmission through haemodialysis and blood transfusions. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |