Egg white-derived peptides prevent cardiovascular disorders induced by mercury in rats: Role of angiotensin-converting enzyme (ACE) and NADPH oxidase
| Autor: | Francisca Fernandez, Dalton Valentim Vassallo, Giulia Alessandra Wiggers, Marta Miguel, Danize Aparecida Rizzetti, Franck Maciel Peçanha, Angela Martín, Patricia Corrales, Maylla Ronacher Simões |
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| Přispěvatelé: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministerio de Economía y Competitividad (España), Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Endothelium Peptidyl-Dipeptidase A 030204 cardiovascular system & hematology Vascular dysfunction Toxicology Superoxide dismutase Phenylephrine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Egg White Internal medicine Renin–angiotensin system medicine Animals Vasoconstrictor Agents Rats Wistar Aorta Egg white hydrolysate NADPH oxidase Dose-Response Relationship Drug biology Chemistry Superoxide NADPH Oxidases Angiotensin-converting enzyme Mercury General Medicine Rats 030104 developmental biology medicine.anatomical_structure Endocrinology Cardiovascular Diseases Oxidative stress Blood pressure biology.protein Endothelium Vascular P22phox Peptides medicine.drug |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0378-4274 |
| Popis: | The study aimed to investigate the effects of egg white hydrolysate (EWH) on vascular disorders induced by mercury (Hg). For this, male Wistar rats were treated for 60 days: Untreated (saline, i.m.); Mercury (HgCl2, i.m., 1st dose 4.6 μg/kg, subsequent doses 0.07 μg/kg/day); Hydrolysate (EWH, gavage, 1 g/kg/day); Hydrolysate-Mercury. Systolic (SBP) and diastolic (DBP) blood pressure measurement and vascular reactivity experiments in aorta were performed. We analyzed endothelial dependent and independent vasodilator responses and vasoconstrictor response to phenylephrine (Phe) in absence and presence of endothelium, a NOS inhibitor, a NADPH oxidase inhibitor, the superoxide dismutase, a non-selective COX inhibitor, a selective COX-2 inhibitor, an AT-1 receptors blocker. In situ superoxide anion production, SOD-1, NOX-4, p22phox, COX-2 and AT-1 mRNA levels and NOX-1 protein expression were performed in aorta while the determination of angiotensin converting enzyme (ACE) activity was measured in plasma. As results, EWH prevented the increase in SBP and Phe responses and the endothelial dysfunction elicited by Hg, which was related to decreased ACE activity and NOX activation by EWH and, subsequently, alleviated ROS production and improved NO bioavailability in aorta. In conclusion, EWH could be considered as alternative or complementary treatment tools for Hg-induced cardiovascular damage. This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico − CNPq [203440/2014-5] and the Ministerio de Economía y Competitividad − MINECO [AGL2012-32387 and CSIC − Intramural201570I028]. |
| Databáze: | OpenAIRE |
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