Collecting high-quality pancreatic tissue for experimental study from organ donors with signs of β-cell autoimmunity

Autor: Immo Rantala, Sisko Tauriainen, Mikael Knip, Heikki Hyöty, Kaija Salmela
Rok vydání: 2010
Předmět:
Male
Insulin Antibodies
Endocrinology
Diabetes and Metabolism
Autoimmunity
medicine.disease_cause
Organ transplantation
0302 clinical medicine
Endocrinology
Insulin-Secreting Cells
Immunopathology
Insulin
Child
Cation Transport Proteins
Finland
0303 health sciences
Glutamate Decarboxylase
Middle Aged
Tissue Donors
3. Good health
medicine.anatomical_structure
Child
Preschool
Tissue and Organ Harvesting
Female
Pancreas Transplantation
Pancreas
Adult
medicine.medical_specialty
Adolescent
030209 endocrinology & metabolism
Zinc Transporter 8
Young Adult
03 medical and health sciences
Internal Medicine
medicine
Humans
Receptor-Like Protein Tyrosine Phosphatases
Class 8
Organ donation
Aged
Autoantibodies
030304 developmental biology
Type 1 diabetes
business.industry
Autoantibody
medicine.disease
Transplantation
Diabetes Mellitus
Type 1
Immunology
business
Zdroj: Diabetes/Metabolism Research and Reviews. 26:585-592
ISSN: 1520-7552
Popis: Background The aim of this study was to create a new research strategy to obtain high-quality pancreatic tissues from subjects with preclinical or clinical type 1 diabetes, which would open up new avenues for studying the mechanisms of the β-cell damaging process in humans. Research design and methods A nationwide collaboration network (the PanFin network) was established in Finland to start an on-call screening of diabetes-associated autoantibodies from deceased organ donors and subsequent processing of pancreases from autoantibody-positive donors. This protocol was integrated into the national organ transplantation procedure. Results Only a few modifications were needed to the normal transplantation practices. One additional blood sample was obtained from donors for autoantibody analyses, the transplantation team was informed about the autoantibody result and the pancreas of autoantibody-positive donors was transported to the core laboratory. Altogether, 307 donors were screened and 22 (7.2%) were positive for at least one autoantibody and 3 tested positive for two or more autoantibodies out of the five tested (islet cell antibodies, insulin autoantibodies and autoantibodies to glutamic acid decarboxylase, islet antigen 2 and zinc transporter 8). The quality of collected pancreatic tissue was superior to that from autopsies and allowed the detection of both RNA and proteins. Conclusions The study protocol was proven feasible to be carried out on a nationwide scale. It did not interfere with the normal transplantation activities and provided valuable tissue material for research. Copyright © 2010 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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