E-cadherin Gene Promoter Hypermethylation in H. pylori-Induced Enlarged Fold Gastritis
| Autor: | Kenji Watabe, Tamana Miyazaki, Yasuhisa Shinomura, Shinji Tamura, Shusaku Tsutsui, Yoko Murayama, Norio Hayashi, Tatsuya Kiyohara, Takahiro Yamamoto |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Biopsy Biology Helicobacter Infections Cell Line Tumor medicine Gastric mucosa Humans Promoter Regions Genetic Aged Helicobacter pylori medicine.diagnostic_test Cadherin Gastroenterology Promoter General Medicine Methylation DNA Methylation Middle Aged Cadherins biology.organism_classification Molecular biology Infectious Diseases medicine.anatomical_structure Gastric Mucosa Gastritis DNA methylation Female medicine.symptom |
| Zdroj: | Helicobacter. 12:523-531 |
| ISSN: | 1523-5378 1083-4389 |
| DOI: | 10.1111/j.1523-5378.2007.00519.x |
| Popis: | Background: Promoter hypermethylation of E-cadherin plays an important role on gastric carcinogenesis. We have previously reported that the odds ratio for gastric carcinoma and the prevalence of diffuse-type early gastric carcinoma in Helicobacter pylori-induced enlarged fold gastritis increased with increasing fold width. Thus, we examined E-cadherin methylation in gastric mucosa from H. pylori-induced enlarged fold gastritis before and after H. pylori eradication. Moreover, we analyzed the mechanism of H. pylori infection-induced E-cadherin hypermethylation. Materials and methods: Twenty-three H. pylori-positive patients with enlarged folds, 18 H. pylori-positive and seven H. pylori-negative patients without enlarged folds, were involved in the study. E-cadherin promoter methylation was studied using quantitative methylation-specific polymerase chain reaction. We investigated methylation percentage and DNA methyltransferase activity in gastric cancer cell lines treated with EGF, TNFα, and MG132. Results: E-cadherin methylation percentage of the gastric antral and body mucosa in H. pylori-positive patients with enlarged folds was much greater than that in both H. pylori-positive and -negative patients without enlarged folds. After H. pylori eradication, the methylation percentage in six patients with enlarged fold gastritis decreased significantly from 15.6 ± 3.9 to 8.8 ± 2.2 (p |
| Databáze: | OpenAIRE |
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