Drosophila chibby is required for basal body formation and ciliogenesis but not for Wg signaling
| Autor: | Joëlle Thomas, Maurice J. Kernan, Brigitte Chhin, Fabien Soulavie, Camille Enjolras, Jean-Luc Duteyrat, Elisabeth Cortier, Bénédicte Durand, Anne Laurençon |
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| Přispěvatelé: | Laboratoire de génétique et physiologie du développement (LGPD), Université de la Méditerranée - Aix-Marseille 2-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut NeuroMyoGène (INMG), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de la Méditerranée - Aix-Marseille 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Centriole external sensory Meckel syndrome [SDV]Life Sciences [q-bio] PNS sound-evoked potential Mice MESH: Sensory Receptor Cells Basal body Drosophila Proteins Wnt Signaling Pathway Cells Cultured Research Articles Centrioles 0303 health sciences biology Cilium NPHP 030302 biochemistry & molecular biology MESH: Spermatozoa Wnt signaling pathway MESH: Wnt Signaling Pathway Nuclear Proteins MESH: Transcription Factors Spermatozoa chordotonal organ MESH: Regulatory Factor X Transcription Factors MESH: Wnt1 Protein Cell biology DNA-Binding Proteins Protein Transport Drosophila melanogaster transition zone Drosophila Protein MESH: Cells Cultured Cell type MESH: Protein Transport Sensory Receptor Cells MESH: Drosophila Proteins Molecular Sequence Data Regulatory Factor X Transcription Factors MESH: Carrier Proteins Wnt1 Protein IFT MESH: Infertility Male Article PLP MESH: Drosophila melanogaster untranslated region 03 medical and health sciences peripheral nervous system MESH: Cilia Ciliogenesis Animals Amino Acid Sequence Cilia MESH: Mice Infertility Male 030304 developmental biology intraflagellar transport MESH: Molecular Sequence Data MESH: Centrioles MKS Cell Biology biology.organism_classification MESH: Male nephronophthisis pericentrin-like protein Carrier Proteins human activities MESH: Nuclear Proteins MESH: DNA-Binding Proteins Transcription Factors |
| Zdroj: | Journal of Cell Biology Journal of Cell Biology, 2012, 197 (2), pp.313-325. ⟨10.1083/jcb.201109148⟩ The Journal of Cell Biology Journal of Cell Biology, Rockefeller University Press, 2012, 197 (2), pp.313-325. ⟨10.1083/jcb.201109148⟩ |
| ISSN: | 0021-9525 1540-8140 |
| DOI: | 10.1083/jcb.201109148⟩ |
| Popis: | In contrast to vertebrate CBY, which functions in WNT signaling, Drosophila CBY is essential for normal basal body structure and function but dispensable for Wg signaling. Centriole-to–basal body conversion, a complex process essential for ciliogenesis, involves the progressive addition of specific proteins to centrioles. CHIBBY (CBY) is a coiled-coil domain protein first described as interacting with β-catenin and involved in Wg-Int (WNT) signaling. We found that, in Drosophila melanogaster, CBY was exclusively expressed in cells that require functional basal bodies, i.e., sensory neurons and male germ cells. CBY was associated with the basal body transition zone (TZ) in these two cell types. Inactivation of cby led to defects in sensory transduction and in spermatogenesis. Loss of CBY resulted in altered ciliary trafficking into neuronal cilia, irregular deposition of proteins on spermatocyte basal bodies, and, consequently, distorted axonemal assembly. Importantly, cby1/1 flies did not show Wingless signaling defects. Hence, CBY is essential for normal basal body structure and function in Drosophila, potentially through effects on the TZ. The function of CBY in WNT signaling in vertebrates has either been acquired during vertebrate evolution or lost in Drosophila. |
| Databáze: | OpenAIRE |
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