Measurement of voltage-gated potassium currents in identified spinally-projecting sympathetic neurones of the paraventricular nucleus
| Autor: | J.H Coote, Richard Barrett-Jolley, S Pyner |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
endocrine system
Sympathetic nervous system Patch-Clamp Techniques Potassium Channels Stilbamidines Central nervous system Efferent Pathways medicine Animals Patch clamp Rats Wistar Fluorescent Dyes Neurons Voltage-gated ion channel Chemistry General Neuroscience digestive oral and skin physiology Tetraethylammonium Spinal cord Potassium channel Rats Electrophysiology medicine.anatomical_structure nervous system Spinal Cord Hypothalamus Adrenergic Fibers Neuroscience hormones hormone substitutes and hormone antagonists Paraventricular Hypothalamic Nucleus |
| Zdroj: | Journal of neuroscience methods. 102(1) |
| ISSN: | 0165-0270 |
| Popis: | The paraventricular nucleus (PVN) of the hypothalamus modulates cardiovascular function via a sub-population of neurones which project directly to sympathetic centres of the spinal cord. Identification and patch-clamp recording from these neurones is difficult, however, because of the complex organisation and neuronal heterogeneity of the PVN. We report here on methods for the in vitro recording of voltage-gated potassium channel (KV) currents from those neurones within the PVN which project to the intermediolateral column of the rat spinal cord, and are believed to directly modulate cardiovascular function. We show KV channel currents of spinally projecting neurones to be slowly inactivating (τ≫100 ms) and weakly sensitive to TEA (Kd>10 mM). These methods will be useful for the study of KV and other ion channel modulation in spinally projecting neurones of the PVN. |
| Databáze: | OpenAIRE |
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