An approach to characterizing uncultivated prokaryotes: the Grey Lung agent and proposal of a Candidatus taxon for the organism, 'Candidatus Mycoplasma ravipulmonis'
| Autor: | Harold Neimark, Ronald H. Leach, Deborah L. Mitchelmore |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Male Immunology Molecular Sequence Data medicine.disease_cause Microbiology law.invention Rodent Diseases chemistry.chemical_compound Mice Mycoplasma law RNA Ribosomal 16S medicine Pneumonia Bacterial Animals Mycoplasma Infections Gene Polymerase chain reaction Genetics Mice Inbred BALB C biology Base Sequence Chromosome 16S ribosomal RNA biology.organism_classification RNA Bacterial chemistry Candidatus Female Bacteria DNA |
| Zdroj: | International journal of systematic bacteriology. |
| ISSN: | 0020-7713 |
| Popis: | An approach to characterizing uncultivated bacteria which combines a PFGE procedure for obtaining purified full-length chromosomes with PCR amplification is described. Isolated chromosomes from uncultivated organisms provide a specifically identifiable source material for hybridization, amplification and cloning. The availability of purified chromosomes for DNA amplification should aid in examining the diversity of microbial populations and should also reduce the possibility of forming hybrid DNA artifact molecules. The approach is illustrated by isolating the chromosome of the uncultivated agent of rodent Grey Lung disease and using the purified chromosomes to amplify and directly sequence the evolutionarily conserved 16S rRNA gene. The Grey Lung agent (GLA) contains a 650 kb chromosome and is shown to be a Mycoplasma sp. located phylogenetically in the hominis group of mycoplasmas. If a simple genomic lesion(s) is responsible for the unculturability of GLA, it is conceivable that complementation with DNA from a close relative could permit growth on artificial media. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|