Immunotoxicity of ethyl-4-isothiocyanatobutanoate in male Wistar rats
| Autor: | Andrea Sovcikova, J. Kubova, A. Lišková, Katarína Horáková, Jana Tulinska |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_specialty Spleen Antineoplastic Agents Biology Toxicology Lymphocyte Activation Immune system In vivo Isothiocyanates Internal medicine Immunopathology White blood cell medicine Leukocytes Animals Rats Wistar Body Weight Immunity Organ Size Primary and secondary antibodies In vitro Rats Butyrates Endocrinology medicine.anatomical_structure Toxicity Immunology Antibody Formation biology.protein |
| Zdroj: | Toxicology. 145(2-3) |
| ISSN: | 0300-483X |
| Popis: | The immunotoxicity of ethyl-4-isothiocyanatobutanoate (E-4IB) using different immuno-pathological parameters and immune function assays in male Wistar rats was evaluated. The rats were administered intraperitoneally 12 times with E-4IB in three varying doses of 21, 28 and 35 mg/kg of body weight, over a period of 36 days. The doses of E-4IB were set according to the results of previous experiments by its anti-proliferative activity in vivo. High and medium doses of E-4IB exceeded the maximum tolerated dose after the 36-day treatment period. Symptoms of toxicity were displayed by a drop in body weight, spleen and thymus weight and in organ and bone marrow cellularity. Haematological changes displayed a dose-dependent decrease in the percentage of lymphocytes and dose-dependent increase in the percentage of polymorphonuclear leukocytes in peripheral blood. The white blood cell count in rats exposed to a high dose of E-4IB was suppressed. The immune system of rats administered 21 mg/kg of E-4IB (low dose) was unaffected. No changes in primary antibody response to sheep erythrocytes, in vitro proliferative response of spleen lymphocytes to mitogens and phagocytic activity of leukocytes were found in those rats. Our findings indicate that this newly developed anti-cancer drug is not immunotoxic. |
| Databáze: | OpenAIRE |
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