Hyperstretching DNA
| Autor: | Schakenraad, Koen, Biebricher, Andreas S., Sebregts, Maarten, Ten Bensel, Brian, Peterman, Erwin J.G., Wuite, Gijs J L, Heller, Iddo, Storm, Cornelis, Van Der Schoot, Paul, Sub Algemeen Theoretical Physics, Theoretical Physics |
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| Přispěvatelé: | Soft Matter and Biological Physics, Institute for Complex Molecular Systems, Physics of Living Systems, LaserLaB - Molecular Biophysics, Physics and Astronomy |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Models Molecular Single Molecule Imaging/methods Chemistry(all) Science General Physics and Astronomy 02 engineering and technology Physics and Astronomy(all) Biochemistry Article General Biochemistry Genetics and Molecular Biology Fluorescence 03 medical and health sciences Computational biophysics Single-molecule biophysics Models Benzoxazoles/chemistry lcsh:Science DNA/chemistry Biomechanical Phenomena/genetics Benzoxazoles Multidisciplinary Base Sequence Biochemistry Genetics and Molecular Biology(all) Quinolinium Compounds Molecular DNA General Chemistry 021001 nanoscience & nanotechnology Base Sequence/genetics Quinolinium Compounds/chemistry Single Molecule Imaging Elasticity Biomechanical Phenomena 030104 developmental biology DNA and RNA Nucleic Acid Conformation lcsh:Q 0210 nano-technology Genetics and Molecular Biology(all) |
| Zdroj: | Nature Communications, 8(2197):2197. Nature Publishing Group Nature Communications, Vol 8, Iss 1, Pp 1-7 (2017) Schakenraad, K, Biebricher, A S, Sebregts, M, ten Bensel, B, Peterman, E J G, Wuite, G J L, Heller, I, Storm, C & van der Schoot, P 2017, ' Hyperstretching DNA ', Nature Communications, vol. 8, no. 1, 2197 . https://doi.org/10.1038/s41467-017-02396-1 Nature Communications Nature Communications, 8(1). Nature Publishing Group Nature Communications, 8(1):2197. Nature Publishing Group |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-017-02396-1 |
| Popis: | The three-dimensional structure of DNA is highly susceptible to changes by mechanical and biochemical cues in vivo and in vitro. In particular, large increases in base pair spacing compared to regular B-DNA are effected by mechanical (over)stretching and by intercalation of compounds that are widely used in biophysical/chemical assays and drug treatments. We present single-molecule experiments and a three-state statistical mechanical model that provide a quantitative understanding of the interplay between B-DNA, overstretched DNA and intercalated DNA. The predictions of this model include a hitherto unconfirmed hyperstretched state, twice the length of B-DNA. Our force-fluorescence experiments confirm this hyperstretched state and reveal its sequence dependence. These results pin down the physical principles that govern DNA mechanics under the influence of tension and biochemical reactions. A predictive understanding of the possibilities and limitations of DNA extension can guide refined exploitation of DNA in, e.g., programmable soft materials and DNA origami applications. The mechanics and structural transitions of DNA are important to many essential processes inside living cells. Here the authors combine theory and single-molecule experiments to show that intercalator binding stabilises a new structural state of DNA: hyperstretched DNA. |
| Databáze: | OpenAIRE |
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