Differential exosome miRNA expression in oral cancer stem cells
| Autor: | D. Harmon, B. Leavitt, T. Booker, M. Shoff, K. Kingsley, K. M. Howard |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer stem cells QH301-705.5 Oral cancer Cell RM1-950 Biology Exosomes Exosome Microvesicles 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Cell culture Cancer stem cell Tumor progression 030220 oncology & carcinogenesis microRNA Cancer research medicine Therapeutics. Pharmacology Biology (General) Stem cell miRNA |
| Zdroj: | ExRNA, Vol 2, Iss 1, Pp 1-9 (2020) |
| ISSN: | 2398-0060 |
| Popis: | Background Oral squamous cell carcinomas (OSCC) secrete exosomes into the surrounding extracellular environment to promote the horizontal transfer of bioactive molecules including microRNA (miRNA). The primary objective of this study was to explore potential differences in miRNA content between OSCC and OSCC stem cells. Methods The OSCC cell lines SCC4, SCC15, SCC25 and CAL27 were used in these studies. The corresponding OSCC stem cells that demonstrated phenotypic adhesion independent tumor spheres (AiTS) were manually isolated. All cells were cultured in DMEM containing 10% exosome-free fetal bovine serum. Exosomes were isolated using Total Exosome Isolation reagent (Invitrogen) and RNA was purified using Total Exosome RNA isolation kit (Invitrogen). Exosome miRNA content was evaluated using miRNA Advanced Taqman Assays for miR-21, − 155, − 133, − 34, − 31, − 32, and − 365. The fold change of miRNA content was calculated using the comparative CT (ΔΔCT) method using miR-16 as an endogenous control. Results After successful cell cultures were established, AiTS (cancer stem cells) were manually separated and confirmed using CD133 and Sox-2 biomarkers. Exosomes and extracellular vesicles were successfully isolated from all cell lines and AiTS isolates for miRNA screening. All isolates exhibited miR-16 expression (positive control), but none contained mir-31, − 32, or 133a. Differential expression of miR-21, miR-34 and miR-155 were observed with patterns observed among the cancer cell lines which were distinct from the corresponding AiTS isolates. Conclusions Exosomes isolated from these different OSCC stem cell populations displayed nearly consistent downregulation/loss of miR-21 and miR-34 suggesting the possibility of a unique miRNA profile characteristic of oral cancer stem cells. These findings highlight the need to investigate the comprehensive functions of miR-21 and miR-34 in tumor progression and continued research to refine a miRNA profile that could aid in distinguishing tumors with poor prognosis. |
| Databáze: | OpenAIRE |
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