Widespread changes in nucleosome accessibility without changes in nucleosome occupancy during a rapid transcriptional induction
| Autor: | Robert E. Kingston, Jakub Mieczkowski, Peggy I. Wang, Sharmistha Kundu, Ruslan I. Sadreyev, Britta Mueller, Michael Y. Tolstorukov |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Line 03 medical and health sciences chemistry.chemical_compound Transcription (biology) Genetics Animals Micrococcal Nuclease Nucleosome Promoter Regions Genetic Enhancer Gene biology Chromosome Mapping Promoter Chromatin Nucleosomes Cell biology Drosophila melanogaster Enhancer Elements Genetic 030104 developmental biology Gene Expression Regulation chemistry Unfolded Protein Response biology.protein DNA Protein Binding Research Paper Developmental Biology Micrococcal nuclease |
| Zdroj: | Genes & Development. 31:451-462 |
| ISSN: | 1549-5477 0890-9369 |
| Popis: | Activation of transcription requires alteration of chromatin by complexes that increase the accessibility of nucleosomal DNA. Removing nucleosomes from regulatory sequences has been proposed to play a significant role in activation. We tested whether changes in nucleosome occupancy occurred on the set of genes that is activated by the unfolded protein response (UPR). We observed no decrease in occupancy on most promoters, gene bodies, and enhancers. Instead, there was an increase in the accessibility of nucleosomes, as measured by micrococcal nuclease (MNase) digestion and ATAC-seq (assay for transposase-accessible chromatin [ATAC] using sequencing), that did not result from removal of the nucleosome. Thus, changes in nucleosome accessibility predominate over changes in nucleosome occupancy during rapid transcriptional induction during the UPR. |
| Databáze: | OpenAIRE |
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