Validation of the EntericBio Panel II® multiplex polymerase chain reaction system for detection of Campylobacter spp., Salmonella spp., Shigella spp., and verotoxigenic E. coli for use in a clinical diagnostic setting
| Autor: | Monika Koziel, Roy D. Sleator, Isabelle O'Callaghan, Dan Corcoran, Brigid Lucey |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Salmonella Verotoxigenic E. coli Biology medicine.disease_cause Sensitivity and Specificity Microbiology Feces Predictive Value of Tests Multiplex polymerase chain reaction medicine Humans Shigella Prospective Studies Shiga-Toxigenic Escherichia coli Campylobacter Enterobacteriaceae Infections General Medicine Predictive value Infectious Diseases Molecular Diagnostic Techniques Predictive value of tests Ireland Multiplex Polymerase Chain Reaction |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 75:46-49 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2012.09.007 |
| Popis: | A total of 717 faeces samples were tested prospectively using the EntericBio Panel II® detection system (Serosep, Limerick, Ireland), in parallel with routine laboratory testing, which combines the EntericBio® system with retrospective culture of each specimen where a target is detected. Discrepancy analysis was conducted using molecular methods. The EntericBio Panel II® assay produced 585 negative and 132 positive results, namely, Campylobacter spp. (n = 66); SLT 1 and/or SLT 2 (n = 64); Salmonella spp. (n = 5); and Shigella spp. (n = 0). Three samples were positive for more than 1 target. Of these results, 4 Campylobacter spp. detections and 4 SLT 1/ SLT 2 detections remained unconfirmed, and the system failed to detect 2 Campylobacter spp. targets detected by routine laboratory detection. The sensitivity, specificity, positive predictive value, negative predictive value, and efficiency were calculated to be 98.4%, 98.7%, 93.9%, 99.7%, and 98.6%, respectively. |
| Databáze: | OpenAIRE |
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