Authentication and Banking of Human Pluripotent Stem Cells
Autor: | Richard Josephson, Jonathan M. Auerbach |
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Rok vydání: | 2009 |
Předmět: |
Pluripotent Stem Cells
Authentication business.industry Cell Guidelines as Topic Cell Biology General Medicine Computational biology Biology Phenotype Cell Line Biotechnology medicine.anatomical_structure Cell culture medicine Blood Banks Humans Epigenetics Stem cell Cell bank business Induced pluripotent stem cell Developmental Biology |
Zdroj: | Current Protocols in Stem Cell Biology |
ISSN: | 1938-8969 1941-7322 |
Popis: | Pluripotent human stem cell lines from embryos or reprogrammed adult cells are not all alike. Cell lines differ widely in their propensity for differentiation, their chromosomal integrity and epigenetic state, immunological profiles, and their availability for research. It is important that all pluripotent cell lines be protected from loss by being properly banked and authenticated, which will also protect current experimental data by enabling its future reproducibility. This unit considers basic guidelines for banking and authentication of pluripotent stem cells that should be easily implementable within any laboratory. Cell Banking is the disciplined preservation of a cell stock in the originally obtained state, as well as stocks representing the baseline state for experimental efforts. Each of these stocks must be authenticated appropriately. Authentication of pluripotent lines verifies five properties: the unique identity of the line, its sterility or freedom from contaminating microorganisms and pathogens, the integrity and stability of its genome, its expression of typical markers of the stem cell phenotype, and its pluripotency upon differentiation. This unit lists and compares several assays to verify each of these stem cell line properties. Thanks to recent advances in molecular biology and the availability of state-of-the-art assays from service providers, the time and material costs of banking and authentication are not excessive for the typical research laboratory. Curr. Protoc. Stem Cell Biol. 11:1C.9.1-1C.9.11. © 2009 by John Wiley & Sons, Inc. Keywords: authentication; cell banking; pluripotent stem cell; working cell bank; master cell bank; identity; sterility; stability; undifferentiated phenotype; pluripotency; GMP; GTP |
Databáze: | OpenAIRE |
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