Effects on hematological parameters, antioxidant and immune responses, AChE, and stress indicators of olive flounders, Paralichthys olivaceus, raised in bio-floc and seawater challenged by Edwardsiella tarda
| Autor: | Saebom Sohn, Su Kyoung Kim, Jun-Hwan Kim, Young Baek Hur |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Aquaculture Aquatic Science Antioxidants Microbiology Superoxide dismutase Fish Diseases 03 medical and health sciences chemistry.chemical_compound Stress Physiological medicine Animals Environmental Chemistry Seawater Edwardsiella tarda Hematologic Tests Environmental Biomarkers biology Paralichthys Enterobacteriaceae Infections 04 agricultural and veterinary sciences General Medicine Glutathione biology.organism_classification Immunity Innate Olive flounder 030104 developmental biology chemistry Catalase Acetylcholinesterase Flatfishes 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Lysozyme |
| Zdroj: | Fish & Shellfish Immunology. 97:194-203 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2019.12.011 |
| Popis: | Studies on the resistance of fish raised in bio-floc systems against bacterial infection are limited. We aimed to evaluate the changes in hematological parameters, antioxidant and immune responses, stress indicators, and acetylcholinesterase (AChE) in olive flounder, Paralichthys olivaceus, raised in bio-floc and seawater for 10 months and, then, infected with Edwardsiella tarda at concentrations of 0 (control), 6.61 × 104, 6.61 × 105, 6.61 × 106, and 6.61 × 107 CFU/g fish for 7 days. The lethal concentration 50% was 4.32 × 107 in bio-floc and 3.11 × 106 in seawater. Hematological parameters were significantly decreased by E. tarda challenge, and plasma components were significantly changed. The superoxide dismutase, catalase, and glutathione-S-transferase activities, as antioxidant responses, were significantly increased after infection, whereas the reduced glutathione level was significantly decreased. The lysozyme activity was significantly increased and the AChE level was significantly decreased after infection. Cortisol and HSP 70, as stress indicators, were also significantly increased. The results indicate that E. tarda infection affected various physiological factors in P. olivaceus. Additionally, P. olivaceus raised in seawater were more susceptible to E. tarda infection than those raised in bio-floc. |
| Databáze: | OpenAIRE |
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