Impact of timing and dosage of a fluoroquinolone treatment on the microbiological, pathological and clinical outcomes of calves challenged with Mannheimia haemolytica
| Autor: | Hervé Cassard, Sébastien Assié, Gilles Meyer, Aude A. Ferran, Maxence Delverdier, Alain Bousquet-Mélou, Frédérique Woerhlé, Guillaume Lhermie, Farid El Garch, Marc Schneider, Diane Pacalin |
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| Přispěvatelé: | Vetoquinol SA, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, LUNAM Université [Nantes Angers Le Mans], ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA), Clinique Vétérinaire, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty medicine.medical_treatment [SDV]Life Sciences [q-bio] 030106 microbiology early treatment bovine model lcsh:QR1-502 Pasteurellaceae decreased regimen fluoroquinolone Biology Gastroenterology Microbiology lcsh:Microbiology 03 medical and health sciences Marbofloxacin Fluoroquinolone Internal medicine medicine Respiratory system Saline Pathological Original Research Lung medicine.disease biology.organism_classification 3. Good health Pneumonia medicine.anatomical_structure Immunology Intramuscular injection medicine.drug |
| Zdroj: | Frontiers in Microbiology, Vol 7 (2016) Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2016, 7, pp.1-13. ⟨10.3389/fmicb.2016.00237⟩ Frontiers in Microbiology (7), 1-13. (2016) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2016.00237/full |
| Popis: | International audience; The efficacy of an early and low inoculum-adjusted marbofloxacin treatment was evaluated on microbiological and clinical outcomes in calves infected with 4.10(7) CFU of Mannheimia haemolytica A1. Twenty-two calves were included based on their rectal temperature rise in the 10 h after challenge and allocated in four groups, receiving a single intramuscular injection of saline (CON), 2 mg/kg marbofloxacin 2-4 h after inclusion (early treatment, E2), 2 or 10 mg/kg marbofloxacin 35-39 h after inclusion (late treatments, L2, L10). In CON calves, M. haemolytica DNA loads in bronchoalveolar lavages continuously increased from inclusion to day 4, and were associated with persistent respiratory clinical signs and lung lesions. At times of early and late treatments, M. haemolytica loads ranged within 3.5-4 and 5.5-6 log10 DNA copies/mL, respectively. Early 2 mg/kg marbofloxacin treatment led to rapid and total elimination of bacteria in all calves. The late treatments induced a reduction of bacterial loads, but 3 of 6 L2 and 1 of 6 L10 calves were still positive for M. haemolytica at day 4. Except for CON calves, all animals exhibited clinical improvement within 24 h after treatment. However, early 2 mg/kg treatment was more efficacious to prevent pulmonary lesions, as indicated by the reduction of the extension and severity of gross lesions and by the histopathological scores. These results demonstrated for the first time that a reduced antibiotic regimen given at an early stage of the disease and targeting a low bacterial load could be efficacious in a natural bovine model of pneumonia. |
| Databáze: | OpenAIRE |
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