A one-step enzyme-linked immunosorbent assay to detect anti-CMV antibodies; development and clinical validation
| Autor: | Charles Dagelinckx, F. Wielaard, Philippe Gausset, Jan C M J. Scherders |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
chemistry.chemical_classification
biology Cytomegalovirus Blood Donors Enzyme-Linked Immunosorbent Assay Antibodies Viral biology.organism_classification Molecular biology Latex fixation test Clinical study Blood donor Enzyme Antigen chemistry Predictive Value of Tests Betaherpesvirinae Virology biology.protein Humans Antibody Antigens Viral Latex Fixation Tests Conjugate |
| Zdroj: | Journal of Virological Methods. 17:141-148 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/0166-0934(87)90077-2 |
| Popis: | A simple one-step ELISA to detect anti-CMV antibodies was developed. The test was based on the inhibition principle, and used anti-CMV coated microtitre plates, CMV nuclear antigen and anti-CMV Fab'-HRP conjugate; total assay time was 1.5 h. The use of Fab'-HRP conjugates improved discrimination between positive and negative sera as compared with IgG-HRP conjugates. In an in-house clinical study, the one-step ELISA showed a 98.4% correlation with the latex agglutination test. The test could be demonstrated to be suitable for both serum and citrate- or EDTA-plasma specimens; heparin-plasma gave somewhat higher absorbance values. In an external clinical validation, the one-step ELISA showed a 99.5% correlation with the latex agglutination test, and was found to be highly suitable for screening of blood donor specimens in a blood bank setting. |
| Databáze: | OpenAIRE |
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