Induction of an anionic peroxidase in cowpea leaves by exogenous salicylic acid
| Autor: | Vadjah C.P. Moraes, Joaquim A. G. Silveira, Jose T.A. Oliveira, Cléberson de Freitas Fernandes, Ilka M. Vasconcelos |
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| Rok vydání: | 2006 |
| Předmět: |
Physiology
Kinetics Plant Science Vigna chemistry.chemical_compound Tobacco mosaic virus Peroxidase chemistry.chemical_classification Chromatography biology Guaiacol Cationic polymerization food and beverages Fabaceae biology.organism_classification Isoenzymes Plant Leaves Enzyme chemistry Biochemistry Enzyme Induction biology.protein Salicylic Acid Agronomy and Crop Science Salicylic acid |
| Zdroj: | Journal of Plant Physiology. 163:1040-1048 |
| ISSN: | 0176-1617 |
| Popis: | Summary Two isoperoxidases were detected in cowpea (Vigna unguiculata) leaves. Treatment of the primary leaves with 10 mM salicylic acid increased the total peroxidase activity contributed by the anionic isoform. To isolate both the anionic and cationic peroxidases the leaf crude extract was loaded on a Superose 12 HR 10/30 column followed by chromatography on Mono-Q HR 5/5. Both enzymes were stable in a pH range from 5 to 7. The optimum-temperatures for the cationic and anionic peroxidase isoforms were, respectively, 20–30 °C and 30 °C. The dependence of guaiacol oxidation rate varying its concentration at constant H2O2 concentration showed, for both enzymes, Michaelis-Menten-type kinetic. Apparent K m s were 0.8 and 4.8 μM for the cationic and anionic isoperoxidases, respectively. |
| Databáze: | OpenAIRE |
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