Molecular emm typing of Bulgarian macrolide-resistant Streptococcus pyogenes isolates
| Autor: | Raina Gergova, Radka Kaneva, Rumyana Markovska, Kalina Mihova, Adile Muhtarova, Ivan Mitov |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Adolescent Genotype Streptococcus pyogenes Population Human pathogen Microbial Sensitivity Tests Biology medicine.disease_cause Group A Microbiology law.invention Young Adult law Streptococcal Infections Drug Resistance Bacterial medicine Humans Typing education Bulgaria Child Polymerase chain reaction Aged Aged 80 and over education.field_of_study General Immunology and Microbiology Streptococcus General Medicine Middle Aged Anti-Bacterial Agents Molecular Typing Phenotype Macrolide resistance Child Preschool Macrolides Bacterial Outer Membrane Proteins |
| Zdroj: | Acta microbiologica et immunologica Hungarica. 67(1) |
| ISSN: | 1588-2640 |
| Popis: | Group A streptococcus (GAS) is a human pathogen causing a broad range of infections, linked with global morbidity and mortality. Macrolide resistance rates vary significantly in different parts of the world. Driving factors of the emergence and spread of resistant clones are not clearly understood. We investigated 102 macrolide-resistant GAS strains collected during the period 2014–2018 from various clinical specimens from Bulgarian patients. Strains were characterized by the presence of mefA/mefE, ermA, and ermB using polymerase chain reaction and sequencing for mefA/mefE. Resistant strains were studied by emm sequence typing and emm-cluster system. Most prevalent emm types among the macrolide-resistant GAS strains were emm28 (22.55%), emm12 (17.65%), and emm4 (16.66%). Almost all (87.25%) of the macrolide-resistant isolates harboring ermB were emm28. The isolates that carried ermA were predominantly emm12 (38.24%) and emm77 (38.24%), with fewer emm89 (23.53%). The isolates harbored predominantly mefE (49 isolates) and only 9 strains carried mefA. The most prevalent emm clusters among the GAS isolates were E4 (40.20%), A-C4 (17.65%), and E1 (16.66%). The study’s results suggest that dissemination of specific clones in GAS population may also be the reason for the increasing macrolide-resistance rate in our country. |
| Databáze: | OpenAIRE |
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