Differential localization of arabinan and galactan side chains of rhamnogalacturonan 1 in cambial derivatives
| Autor: | Christian Cibert, Claudine Morvan, Brigitte Vian, Fabienne F. Ermel, Renée Goldberg, Anne-Marie Catesson, Marie-Laure Follet-Gueye |
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| Rok vydání: | 2000 |
| Předmět: |
Time Factors
food.ingredient Pectin Fluorescent Antibody Technique Plant Science Biology Galactans Trees Cell wall chemistry.chemical_compound food Cell Wall Polysaccharides Botany Genetics Cambium Microscopy Immunoelectron Microscopy Confocal food and beverages Xylem Immunogold labelling Galactan chemistry Biophysics Pectins Phloem Plant Structures Cell wall thickening |
| Zdroj: | Planta. 210:732-740 |
| ISSN: | 1432-2048 0032-0935 |
| Popis: | The development of pectin structural features during the differentiation of cambial derivatives was investigated in aspen (Populus tremula L. x P. tremuloides Michx.) using biochemical and immunocytochemical methods. Comparisons were also made between active and resting tissues. Active tissues, in particular cambial cells and phloem derivatives, were characterized by a high pectin content. Use of antibodies raised against arabinan side chains of rhamnogalacturonan 1 (LM6), as well as biochemical analysis, revealed an obvious decrease from the cortex to the differentiating xylem. Galactan side chains, detected with LM5 antibodies, were present mainly in the cambial zone and enlarging xylem cells. In contrast, they were totally absent from sieve-tube cell walls. Image analysis of LM5 immunogold labelling in the cambial zone showed a clustered distribution of galactan epitopes in the radial walls, a distribution which might result from the association of two different periodic processes, namely the exocytosis of galactan and wall expansion. Cessation of cambial activity was characterized by cell wall thickening accompanied by a sharp decrease in the relative amount of pectin and a lowering of the degree of methylesterification. The data provide evidence that the walls of phloem and xylem cells differ in their pectin composition even at a very early stage of commitment. These differences offer useful tools for identifying the initial cells among their immediate neighbours. |
| Databáze: | OpenAIRE |
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