The role of potassium channels and calcium in the relaxation mechanism of magnesium sulfate on the isolated rat uterus
| Autor: | Dragana Sokolović, Duško Blagojević, Zorana Oreščanin-Dušić, Nikola Tatalović, Milica Pecelj, Slobodan Milovanović, Dragana Drakul |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Tocolytic agent tocolytic chemistry.chemical_element Calcium Pharmacology General Biochemistry Genetics and Molecular Biology Glibenclamide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine lcsh:QH301-705.5 Tetraethylammonium uterus Magnesium Calcium channel Uterus fungi Antagonist equipment and supplies Potassium channel MgSO4 030104 developmental biology chemistry lcsh:Biology (General) Tocolytic K+ channels Ca2+ channels General Agricultural and Biological Sciences 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Archives of Biological Sciences, Vol 71, Iss 1, Pp 5-11 (2019) Archives of Biological Sciences |
| ISSN: | 1821-4339 0354-4664 |
| Popis: | MgSO 4 is used as a tocolytic agent. It is considered to be a calcium channel antagonist, but a different mechanism of its action might be involved. The aim of this study was to examine the contribution of calcium concentrations and potassium channels in the mechanism of MgSO 4 -mediated uterine relaxation. Isolated uteri from female Wister rats were treated with increasing MgSO 4 concentrations (0.1-30 mM). MgSO 4 induced dose-dependent inhibition of spontaneous activity. Addition of Ca 2+ (6 mM and 12 mM) stimulated uterine contractile activity and attenuated the inhibitory activity of MgSO 4 . In order to analyze the role of different subtypes of potassium channels, Ca 2+ -stimulated uteri were pretreated with glibenclamide (Glib), a selective ATP-sensitive potassium channel inhibitor (K ATP ), tetraethylammonium (TEA), a non-specific inhibitor of large conductance calcium-activated potassium channels (BK Ca ), and 4-aminopyridine (4-AP), a voltage-sensitive potassium channel inhibitor (Kv), at concentrations that had no effect per se . Pretreatment with 4-AP had no effect on MgSO 4 -mediated relaxation of Ca 2+ -stimulated uteri. The relaxing effect of MgSO 4 was potentiated by pretreatment with glibenclamide. Pretreatment with TEA attenuated the MgSO 4 -mediated decrease in frequency. Our results suggest that MgSO 4 acts as a general calcium antagonist that influences Ca 2+ -mediated potassium channels. Furthermore, it seems that MgSO 4 uterine relaxation activity is partially mediated by selective ATP-sensitive potassium channels, suggesting an ATP-dependent role. https://doi.org/10.2298/ABS180615031S Received: June 15, 2018; Revised: July 6, 2018; Accepted: July 6, 2018; Published online: July 16, 2018 How to cite this article: Sokolovic D, Drakul D, Orescanin-Dusic Z, Tatalovic N, Pecelj M, Milovanovic S, Blagojevic D. The role of potassium channels and calcium in the relaxation mechanism of magnesium sulfate on the isolated rat uterus. Arch Biol Sci. 2019;71(1):5-11. |
| Databáze: | OpenAIRE |
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