Octanoate Attenuates Adipogenesis in 3T3-L1 Preadipocytes

Autor: Tamar Pirtskhalava, Stephen R. Farmer, Barbara E. Corkey, Wen Guo, James L. Kirkland, Jianrong Han, Yasuo Ido, Richard Yoon
Rok vydání: 2002
Předmět:
Leptin
medicine.medical_specialty
Blotting
Western
Receptors
Cytoplasmic and Nuclear
Medicine (miscellaneous)
Peroxisome proliferator-activated receptor
Glycerolphosphate Dehydrogenase
Nerve Tissue Proteins
Biology
Fatty Acid-Binding Proteins
Fatty acid-binding protein
Mice
chemistry.chemical_compound
Internal medicine
Adipocyte
Adipocytes
medicine
Animals
RNA
Messenger
chemistry.chemical_classification
Nutrition and Dietetics
Calcium-Binding Proteins
Membrane Proteins
Troglitazone
Cell Differentiation
3T3-L1
3T3 Cells
Lipid Metabolism
Growth Inhibitors
Neoplasm Proteins
Sterol regulatory element-binding protein
DNA-Binding Proteins
Repressor Proteins
Endocrinology
Adipose Tissue
chemistry
Adipogenesis
CCAAT-Enhancer-Binding Proteins
Intercellular Signaling Peptides and Proteins
Ectopic expression
Caprylates
Carrier Proteins
Fatty Acid-Binding Protein 7
Sterol Regulatory Element Binding Protein 1
Transcription Factor CHOP
Transcription Factors
medicine.drug
Zdroj: The Journal of Nutrition. 132:904-910
ISSN: 0022-3166
DOI: 10.1093/jn/132.5.904
Popis: Preadipocytes exposed to octanoate accumulate less lipid than cells exposed to long-chain fatty acids. This effect of octanoate involves significant attenuation of expression of key adipogenic transcription factors, including peroxisome proliferator-activated receptor (PPAR)gamma, steroid regulatory binding element protein (SREBP)-1c and CCAAT element binding protein (C/EBPalpha) at both the mRNA and protein levels. Expression of differentiation markers, including adipocyte fatty acid binding protein (ALBP), glycerol-3-phosphate dehydrogenase (GPDH) and leptin, was also significantly diminished by octanoate. However, octanoate did not prevent the decrease in preadipocyte factor-1 (Pref-1) expression that occurs during adipogenesis, nor did it inhibit the early induction of C/EBPbeta,delta. Treatment with synthetic PPARgamma ligands partially offset the inhibitory effect of octanoate on differentiation. Ectopic expression of PPARgamma2 in 3T3-L1 cells partially restored lipid accretion and GPDH activity in octanoate-treated cells. Adding octanoate together with troglitazone attenuated the effects of troglitazone on adipocyte differentiation in both normal 3T3-L1 cells and engineered 3T3-L1 cells that expressed ectopic PPARgamma2, implying that octanoate might compete against troglitazone for its binding to PPARgamma. These results suggest that octanoate may block adipogenesis at least in part by its influence on the expression/activation of PPARgamma.
Databáze: OpenAIRE
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