Association of terpinolene and diclofenac presents antinociceptive and anti-inflammatory synergistic effects in a model of chronic inflammation

Autor: Francisco de Assis Oliveira, Francisco Valmor Macedo Cunha, W.C. Santos, Everton Moraes Lopes, Damião Pergentino de Sousa, E.M.A. Macedo, B.P. Sousa Neto, Celyane Alves Piauilino, Fernanda R.C. Almeida
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Medicine (General)
Time Factors
Physiology
Freund's Adjuvant
Pharmacology
Biochemistry
Antinociception
0302 clinical medicine
Oral administration
Edema
Biology (General)
General Pharmacology
Toxicology and Pharmaceutics
lcsh:QH301-705.5
Pain Measurement
Analgesics
lcsh:R5-920
General Neuroscience
Anti-Inflammatory Agents
Non-Steroidal
Stomach
Drug Synergism
General Medicine
Pharmacological synergism
Terpinolene
Drug Combinations
Treatment Outcome
Hyperalgesia
Female
medicine.symptom
lcsh:Medicine (General)
medicine.drug
Diclofenac
QH301-705.5
medicine.drug_class
Immunology
Analgesic
Biophysics
Pain
Ocean Engineering
Cyclohexane Monoterpenes
Anti-inflammatory
03 medical and health sciences
R5-920
Randall–Selitto test
medicine
Animals
Rats
Wistar
Inflammation
business.industry
Terpenes
Reproducibility of Results
Biomedical Sciences
Cell Biology
030104 developmental biology
Mechanism of action
lcsh:Biology (General)
Freund's adjuvant
Chronic Disease
business
030217 neurology & neurosurgery
Zdroj: Brazilian Journal of Medical and Biological Research, Volume: 49, Issue: 7, Article number: e5103, Published: 20 JUN 2016
Brazilian Journal of Medical and Biological Research, Vol 49, Iss 7 (2016)
Brazilian Journal of Medical and Biological Research v.49 n.7 2016
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Popis: Pharmacological treatment of inflammatory pain is usually done by administration of non-steroidal anti-inflammatory drugs (NSAIDs). These drugs present high efficacy, although side effects are common, especially gastrointestinal lesions. One of the pharmacological strategies to minimize such effects is the combination of drugs and natural products with synergistic analgesic effect. The monoterpene terpinolene (TPL) is a chemical constituent of essential oils present in many plant species, which have pharmacological activities, such as analgesic and anti-inflammatory. The association of ineffective doses of TPL and diclofenac (DCF) (3.125 and 1.25 mg/kg po, respectively) presented antinociceptive and anti-inflammatory effects in the acute (0, 1, 2, 3, 4, 5 and 6 h, after treatment) and chronic (10 days) inflammatory hyperalgesia induced by Freund's complete adjuvant (CFA) in the right hind paw of female Wistar rats (170-230 g, n=6-8). The mechanical hyperalgesia was assessed by the Randall Selitto paw pressure test, which determines the paw withdrawal thresholds. The development of edema was quantified by measuring the volume of the hind paw by plethismography. The TPL/DCF association reduced neutrophils, macrophages and lymphocytes in the histological analysis of the paw, following a standard staining protocol with hematoxylin and eosin and the counts were performed with the aid of optical microscopy after chronic oral administration of these drugs. Moreover, the TPL/DCF association did not induce macroscopic gastric lesions. A possible mechanism of action of the analgesic effect is the involvement of 5-HT2A serotonin receptors, because ketanserin completely reversed the antinociceptive effect of the TPL/DCF association. These results suggest that the TPL/DCF association had a synergistic anti-inflammatory and analgesic effect without causing apparent gastric injury, and that the serotonergic system may be involved in the antinociceptive effect of this association.
Databáze: OpenAIRE
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