Utilization of soluble fusion proteins for induction of T cell proliferation

Autor: D.A. Kirschmann, Christine P. Bono, Michelle L. Zacheis, Benjamin D. Schwartz, Susan L. Woulfe, P. A. De Ciechi
Rok vydání: 1995
Předmět:
musculoskeletal diseases
Monosaccharide Transport Proteins
T cell
Recombinant Fusion Proteins
T-Lymphocytes
Immunology
Antigen presentation
Molecular Sequence Data
Hemagglutinin (influenza)
Hemagglutinins
Viral
Peptide
Peptide binding
Enzyme-Linked Immunosorbent Assay
Hemagglutinin Glycoproteins
Influenza Virus
Lymphocyte Activation
Maltose-Binding Proteins
Maltose-binding protein
Bacterial Proteins
medicine
Escherichia coli
Tetanus Toxoid
Humans
Amino Acid Sequence
Peptide sequence
Cells
Cultured
Cell Line
Transformed
HLA-DR Serological Subtypes
chemistry.chemical_classification
Antigen Presentation
B-Lymphocytes
HLA-D Antigens
biology
Base Sequence
Escherichia coli Proteins
HLA-DR Antigens
Molecular biology
Fusion protein
Peptide Fragments
medicine.anatomical_structure
chemistry
Periplasmic Binding Proteins
biology.protein
ATP-Binding Cassette Transporters
Carrier Proteins
Zdroj: Cellular immunology. 160(2)
ISSN: 0008-8749
Popis: A peptide display library was evaluated as a means to identify peptide binding motifs for class II molecules. Peptides expressed as part of a soluble fusion protein with a maltose binding protein (malE) were produced by Escherichia coli. Constructs containing the high-affinity binding influenza hemagglutinin peptide 307W-319 (mal-HA) or the low-affinity binding tetanus toxoid peptide 830-843 (mal-TT) were used as controls. mal-HA, but not mal-TT, inhibited synthetic biotinylated-HA peptide from binding to purified DR4 Dw4 molecules in a dose-dependent manner. The fusion-peptide presentation system was also evaluated for its ability to induce antigen-specific T cell proliferation. DR4 Dw4+ B cells pulsed with mal-HA, but not mal-TT, induced dose-dependent proliferation of an HA-specific DR4 Dw4-restricted T cell line to the same extent as synthetic HA peptide. Using this type of peptide display library, it may be possible to determine the antigenic specificity of T cell clones isolated from patients with autoimmune diseases.
Databáze: OpenAIRE
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