Effects of α-tocopherol on hemolysis and oxidative stress markers on red blood cells in β-thalassemia major
| Autor: | Mohammad Sadikin, Djajadiman Gatot, Pustika Amalia Wahidiyat, Franciscus D. Suyatna, Munar Lubis, Saptawati Bardosono, Nora Sovira |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Ineffective erythropoiesis medicine.medical_specialty Antioxidant medicine.medical_treatment medicine.disease_cause Pediatrics 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine oxidative stress α-tocopherol biology business.industry Haptoglobin Erythrocyte fragility lcsh:RJ1-570 lcsh:Pediatrics Glutathione Hematology β-thalassemia major Malondialdehyde medicine.disease Hemolysis 030104 developmental biology Endocrinology chemistry 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health biology.protein Original Article hemolysis business Oxidative stress |
| Zdroj: | Clinical and Experimental Pediatrics, Vol 63, Iss 8, Pp 314-320 (2020) Clinical and Experimental Pediatrics |
| ISSN: | 2713-4148 |
| Popis: | Background: The accumulation of unpaired α-globin chains in patients with β-thalassemia major may clinically create ineffective erythropoiesis, hemolysis, and chronic anemia. Multiple blood transfusions and iron overload cause cellular oxidative damage. However, α-tocopherol, an antioxidant, is a potent scavenger of lipid radicals in the membranes of red blood cells (RBCs) of patients with β-thalassemia major.Purpose: To evaluate the effects of α-tocopherol on hemolysis and oxidative stress markers on the RBC membranes of patients with β-thalassemia major.Methods: Forty subjects included in this randomized controlled trial were allocated to the placebo and α-tocopherol groups. Doses of α-tocopherol were based on Institute of Medicine recommendations: 4–8 years old, 200 mg/day; 9–13 years old, 400 mg/day; 14–18 years old, 600 mg/day. Hemolysis, oxidative stress, and antioxidant variables were evaluated before and after 4-week α-tocopherol or placebo treatment, performed before blood transfusions.Results: Significant enhancements in plasma haptoglobin were noted in the α-tocopherol group (3.01 mg/dL; range, 0.60–42.42 mg/dL; P=0.021). However, there was no significant intergroup difference in osmotic fragility test results; hemopexin, malondialdehyde, reduced glutathione (GSH), or oxidized glutathione (GSSG) levels; or GSH/GSSG ratio.Conclusion: Use of α-tocopherol could indirectly improve hemolysis and haptoglobin levels. However, it played no significant role in oxidative stress or as an endogen antioxidant marker in β-thalassemia major. |
| Databáze: | OpenAIRE |
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