Effects of hyperbaric oxygen on hippocampal neuronal apoptosis in rats with acute carbon monoxide poisoning
| Autor: | Jinsheng Li, Jun-Xiang Bao, Xue Gong, Xiao-Ping Xie, Ya Li, Yao-Ming Chang, Li Xue, Wenlan Wang, Hai-Jun Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
Apoptosis Hippocampal formation Hippocampus Rats Sprague-Dawley Andrology Carbon Monoxide Poisoning Random Allocation 03 medical and health sciences 0302 clinical medicine Hyperbaric oxygen Western blot medicine Animals Hippocampus (mythology) Neurons Hyperbaric Oxygenation medicine.diagnostic_test Caspase 3 Carbon monoxide poisoning business.industry General Medicine medicine.disease Rats Staining Enzyme Activation Matrix Metalloproteinase 9 030220 oncology & carcinogenesis Immunohistochemistry business 030217 neurology & neurosurgery |
| Zdroj: | Undersea and Hyperbaric Medicine. 44:121-131 |
| ISSN: | 1066-2936 |
| DOI: | 10.22462/3.4.2017.5 |
| Popis: | Introduction: Acute carbon monoxide (CO) poisoning causes serious health problems such as neuropsychological sequelae. This study aimed to investigate neuronal apoptosis and the effects of hyperbaric oxygen (HBO2) on different regions of the rat hippocampus after CO poisoning. Methods: 90 mature male Sprague Dawley rats were randomly divided into three groups: the normal control group (NC group), the acute carbon monoxide-poisoned group (CO group) and the hyperbaric oxygen treatment group (HBO2 group). CO exposure included 0, 1, 3, 7, 14 and 21 treatment days, one exposure on the first day, and sacrifice on each of the following days. HBO2 exposure included treatment for 0, 1, 3, 7, 14 and 21 days, daily treatment after CO exposure, and sacrifice after the last HBO2 treatment on each of those days. Hematoxylin-eosin staining, immunohistochemical staining, immunofluorescence staining, and western blot analysis were performed to detect apoptosis in brain tissue samples. Results: MMP-9 and caspase-3 were prominently increased by CO exposure and inhibited by HBO2 in the CA3 region in the hippocampus at one, three and seven days (immunohistochemical staining [IHC]: P < 0.05). Neu N and the ratio of Bcl-2/ BAX were prominently decreased by CO exposure and rescued by HBO2 in the CA3 region after seven days of treatment (IHC: P < 0.05). Conclusion: These findings indicated that neuronal apoptosis in the rat hippocampus could be induced by acute CO exposure, especially in the CA3 region. HBO2 could effectively inhibit neuronal apoptosis, especially in the CA3 region after seven days of treatment. The application of HBO2 to inhibit MMP-9 and apoptosis may contribute to brain recovery after acute CO poisoning. |
| Databáze: | OpenAIRE |
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