Purification and Characterization of a Central Cholinergic Enhancing Factor from Rat Brain: Its Identity as Phosphoethanolamine
| Autor: | Diane W. Landers, Kenneth Lau, J. Robert Bostwick, Stanley H. Appel, Garrett Crawford |
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| Rok vydání: | 1989 |
| Předmět: |
Biology
Biochemistry Choline O-Acetyltransferase Cellular and Molecular Neuroscience chemistry.chemical_compound Ethanolamine Culture Techniques medicine Animals Choline Cholinergic neuron Medial septal nucleus Rats Inbred Strains Cytidine Choline acetyltransferase Acetylcholine Stimulation Chemical Rats Molecular Weight medicine.anatomical_structure chemistry Ethanolamines Cholinergic Septal Nuclei Cell Division medicine.drug |
| Zdroj: | Journal of Neurochemistry. 53:448-458 |
| ISSN: | 1471-4159 0022-3042 |
| Popis: | A compound that can enhance the apparent synthesis of acetylcholine in cultured explants of the medial septal nucleus has been purified from rat brain and identified as phosphoethanolamine. Acetylcholine synthesis is stimulated two- to threefold in cultures grown for 5 days in the presence of phosphoethanolamine, ethanolamine, or cytidine 5'-diphosphoethanolamine at concentrations above 100 microM. This effect appears to result from an increase in the accumulation of choline via the high-affinity, sodium-dependent uptake mechanism. The development of choline acetyltransferase activity is not affected. Phosphoethanolamine and ethanolamine seem to enhance the ability of developing cholinergic neurons to utilize choline accumulated via the sodium-dependent high-affinity choline uptake mechanism for the preferential production of acetylcholine without increasing the general metabolism of the cultures. Choline itself and its related derivatives are not stimulatory for these effects. |
| Databáze: | OpenAIRE |
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