Transglutaminase-mediated crosslinking of Bambara groundnut protein hydrogels: Implications on rheological, textural and microstructural properties
Autor: | Tukayi Kudanga, Faith Matiza Ruzengwe, Eric O. Amonsou |
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Rok vydání: | 2020 |
Předmět: |
chemistry.chemical_classification
0303 health sciences Transglutaminases biology 030309 nutrition & dietetics Chemistry Tissue transglutaminase G protein Hydrogels 04 agricultural and veterinary sciences 040401 food science 03 medical and health sciences 0404 agricultural biotechnology Chemical engineering Rheology Homogeneous Plant protein Hardness Self-healing hydrogels biology.protein Thiol Amine gas treating Sulfhydryl Compounds Food Science |
Zdroj: | Food research international (Ottawa, Ont.). 137 |
ISSN: | 1873-7145 |
Popis: | Interest in plant protein-based hydrogels with desirable strength has been increasing in recent years. In this study, Bambara groundnut protein isolate (BPI) was crosslinked with transglutaminase (TGase) (0 – 25 U/g protein) during gelation and rheological, textural and microstructural properties of the resulting hydrogels were investigated. Treatment with TGase up to 15 U/g protein resulted in the formation of hydrogels with small pores and an organised homogeneous network. G’ of TGase-treated BPI hydrogels was more than ten-fold higher than G” throughout the frequency range of 0–100 rad/s, suggesting dominance of the elastic like behaviour. BPI hydrogel with the highest G’ (6967 Pa) and hardness (5.60 N) was formed at 15 U/g protein of TGase activity. The hydrogel had a high distribution β-sheets (53.52%) and α-helixes (26.17%) as compared to the β-turns and random coils. However, a further increase in TGase activity did not improve the hydrogel properties. Transglutaminase mediated crosslinking of BPI hydrogel was demonstrated by the reduction in amine and thiol groups and the formation of a new protein band (56 kDa) in crosslinked hydrogels. Overall, TGase promoted the formation of a strong gel with an organised network. |
Databáze: | OpenAIRE |
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