A novel type of DNA-binding protein interacts with a conserved sequence in an early nodulin ENOD12 promoter
| Autor: | Kjeld A. Marcker, Erik Østergaard Jensen, Wei-Cai Yang, Ton Bisseling, Knud Larsen, Irma Vijn, Niels Pallisgaard, Henning Christiansen, Anette Chemnitz Hansen |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary root nodule DNA Plant Molecular Sequence Data Response element Gene Expression Plant Science Biology medicine.disease_cause DNA-binding protein cis element Rhizobium leguminosarum Homology (biology) Conserved sequence Complementary DNA AT hook Genetics medicine Animals Laboratorium voor Moleculaire Biologie Amino Acid Sequence Promoter Regions Genetic Transcription factor Gene Plant Proteins Binding Sites Plants Medicinal Base Sequence Sequence Homology Amino Acid Membrane Proteins food and beverages Fabaceae General Medicine trans acting factor Molecular biology ENOD12 Rats DNA-Binding Proteins nodulin Promoter Regions (Genetics) Laboratory of Molecular Biology EPS Agronomy and Crop Science Transcription Factors |
| Zdroj: | Plant Molecular Biology, 32, 809-821 Plant Molecular Biology 32 (1996) Christiansen, H, Hansen, A C, Vijn, I, Pallisgaard, N, Larsen, K, Yang, W C, Bisseling, T, Marcker, K A & Jensen, E Ø 1996, ' A novel type of DNA-binding protein interacts with a conserved sequence in an early nodulin ENOD12 promoter. ', Plant Molecular Biology, vol. 32, no. 5, pp. 809-21 . |
| ISSN: | 0167-4412 |
| DOI: | 10.1007/bf00020479 |
| Popis: | The pea genes PsENOD12A and PsENOD12B are expressed in the root hairs shortly after infection with the nitrogen-fixing bacterium Rhizobium leguminosarum bv. viciae or after application of purified Nod factors. A 199 bp promoter fragment of the PsENOD12B gene contains sufficient information for Nod factor-induced tissue-specific expression. We have isolated a Vicia sativa cDNA encoding a 1641 amino acid protein, ENBP1, that interacts with the 199 bp ENOD12 promoter. Two different DNA-binding domains were identified in ENBP1. A domain containing six AT-hooks interacts specifically with an AT-rich sequence located between positions -95 and -77 in the PsENOD12B promoter. A second domain in ENBP1 is a cysteine-rich region that binds to the ENOD12 promoter in a sequence non-specific but metal-dependent way. ENBP1 is expressed in the same cell types as ENOD12. However, additional expression is observed in the nodule parenchyma and meristem. The presence of three small overlapping ORFs in the 5'-untranslated region of the ENBP1 cDNA indicates that ENBP1 expression might be regulated at the translational level. The interaction of ENBP1 with a conserved AT-rich element within the ENOD12 promoter and the presence of the ENBP1 transcript in cells expressing ENOD12 strongly suggest that ENBP1 is a transcription factor involved in the regulation of ENOD12. Finally, the C-terminal region of ENBP1 shows strong homology to a protein from rat that is specifically expressed in testis tissue. Udgivelsesdato: 1996-Dec |
| Databáze: | OpenAIRE |
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