Comparative analysis of the alveolar microbiome in COPD, ECOPD, Sarcoidosis, and ILD patients to identify respiratory illnesses specific microbial signatures
| Autor: | Martin S. Mortensen, Gaura Chaturvedi, Nitin Goel, Agrima Sharma, Mitali Mukerji, Monika Yadav, Shashank Gupta, Søren J. Sørensen, Nar Singh Chauhan, Malini Shariff |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Lung microbiome Sarcoidosis Science Microbial communities Disease Bronchoalveolar Lavage Article Diagnosis Differential Pulmonary Disease Chronic Obstructive 03 medical and health sciences 0302 clinical medicine Microbial ecology RNA Ribosomal 16S medicine Humans Microbiome Respiratory system Symbiosis Lung Aged COPD Multidisciplinary Bacteria biology business.industry Microbiota Fusobacteria Middle Aged respiratory system medicine.disease biology.organism_classification 030104 developmental biology medicine.anatomical_structure 030228 respiratory system Immunology Medicine Female Metagenomics Lung Diseases Interstitial business |
| Zdroj: | Gupta, S, Shariff, M, Chaturvedi, G, Sharma, A, Goel, N, Yadav, M, Mortensen, M S, Sørensen, S J, Mukerji, M & Chauhan, N S 2021, ' Comparative analysis of the alveolar microbiome in COPD, ECOPD, Sarcoidosis, and ILD patients to identify respiratory illnesses specific microbial signatures ', Scientific Reports, vol. 11, 3963 . https://doi.org/10.1038/s41598-021-83524-2 Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-83524-2 |
| Popis: | Studying respiratory illness-specific microbial signatures and their interaction with other micro-residents could provide a better understanding of lung microbial ecology. Each respiratory illness has a specific disease etiology, however, so far no study has revealed disease—specific microbial markers. The present study was designed to determine disease-specific microbial features and their interactions with other residents in chronic obstructive pulmonary diseases (stable and exacerbated), sarcoidosis, and interstitial lung diseases. Broncho-alveolar lavage samples (n = 43) were analyzed by SSU rRNA gene sequencing to study the alveolar microbiome in these diseases. A predominance of Proteobacteria followed by Firmicutes, Bacteroidetes, Actinobacteria, and Fusobacteria was observed in all the disease subsets. Shannon diversity was significantly higher in stable COPD when compared to exacerbated chronic obstructive pulmonary disease (ECOPD) (p = 0.0061), and ILD patient samples (p = 0.037). The lung microbiome of the patients with stable COPD was more diverse in comparison to ECOPD and ILD patients (p 4). Species network analysis indicated a significant correlation (p |
| Databáze: | OpenAIRE |
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