Hyaluronic Acid Promotes Differentiation of Mesenchymal Stem Cells from Different Sources toward Pancreatic Progenitors within Three-Dimensional Alginate Matrixes
| Autor: | Albert Espona-Noguera, Alberto Cañibano-Hernández, Jesús Ciriza, José Luis Pedraz, Gorka Orive, Rosa Maria Hernandez, Laura Saenz del Burgo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Alginates
Cell Survival medicine.medical_treatment Cellular differentiation Pharmaceutical Science 02 engineering and technology 030226 pharmacology & pharmacy Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine parasitic diseases Drug Discovery Hyaluronic acid medicine Animals Insulin cardiovascular diseases Hyaluronic Acid Progenitor cell Pancreas Cells Cultured Mice Inbred BALB C geography geography.geographical_feature_category Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Islet Cell biology Transplantation Cellular Microenvironment chemistry Molecular Medicine Stem cell 0210 nano-technology |
| Zdroj: | Molecular Pharmaceutics. 16:834-845 |
| ISSN: | 1543-8392 1543-8384 |
| DOI: | 10.1021/acs.molpharmaceut.8b01126 |
| Popis: | Islet transplantation has shown to be a successful alternative in type 1 diabetes treatment, but donor scarcity precludes its worldwide clinical translation. Stem cells are an unlimited source that could circumvent the lack of donors if complete differentiation into insulin-producing cells (IPCs) could be accomplished. We have performed the differentiation of mesenchymal stem cells (MSCs) from different sources into IPCs within three-dimensional (3D) alginate matrixes. We quantified an increased insulin release at the final stage of differentiation compared to undifferentiated MSCs, which is more pronounced in IPCs differentiated from pancreatic-derived MSCs tissues. Moreover, the addition of hyaluronic acid (HA) in alginate microcapsules enhanced, even more, the insulin release from the final IPCs, independent of the MSC source. We can conclude that MSCs can be differentiated into IPCs within alginate microcapsules, enhancing insulin release when HA is present in the 3D alginate matrixes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|