Stereoselective determination of venlafaxine and its three demethylated metabolites in human plasma and whole blood by liquid chromatography with electrospray tandem mass spectrometric detection and solid phase extraction
| Autor: | Johan Ahlner, Martin Josefsson, Finn Bengtsson, Maria Kingbäck, Louise Karlsson, Fredrik C. Kugelberg, Björn Carlsson |
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| Rok vydání: | 2010 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray Metabolite Clinical Biochemistry Venlafaxine Hydrochloride Pharmaceutical Science Mass spectrometry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Drug Stability Liquid chromatography–mass spectrometry Desvenlafaxine Succinate Drug Discovery Humans Solid phase extraction Spectroscopy Whole blood Chromatography Solid Phase Extraction Stereoisomerism Cyclohexanols chemistry Selective Serotonin Reuptake Inhibitors Chromatography Liquid |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 53:583-590 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2010.03.043 |
| Popis: | A stereoselective method is described for simultaneous determination of the S- and R-enantiomers of venlafaxine and its three demethylated metabolites in human plasma and whole blood samples. This validated method involved LC/MS/MS with positive electrospray ionization and solid phase extraction. Chromatographic separation was performed on a 250 mm x 2.1mm Chirobiotic V column with a total run time of 35 min. In plasma, calibration curves were in the range of 1-1000 nM for the S- and R-enantiomers of venlafaxine and O-desmethylvenlafaxine, and 0.5-500 nM for N-desmethylvenlafaxine and N,O-didesmethylvenlafaxine. In whole blood the corresponding concentrations were 10-4000 and 5-2000 nM, respectively. The intra-day precision was6.3% and the inter-day precision was9.9% for plasma and15% and19% for whole blood. LLOQ ranged between 0.25 and 0.5 nM. No ion suppression/enhancement or other matrix effects were observed. The method was successfully applied for determination of venlafaxine and its metabolites in plasma from patients and whole blood samples from forensic autopsy cases. |
| Databáze: | OpenAIRE |
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