Loss of citron kinase affects a subset of progenitor cells that alters late but not early neurogenesis in the developing rat retina
| Autor: | Nisarg Chhaya, Amye Black, Christopher Lemoine, Sean Congdon, Devi Krishna Priya Karunakaran, Rahul N. Kanadia |
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| Rok vydání: | 2015 |
| Předmět: |
Retinal Ganglion Cells
Cellular differentiation Neurogenesis Biology Protein Serine-Threonine Kinases Retina Cellular and Molecular Neuroscience Pregnancy medicine In Situ Nick-End Labeling Animals Progenitor cell Rats Wistar TUNEL assay Reverse Transcriptase Polymerase Chain Reaction Stem Cells Intracellular Signaling Peptides and Proteins Gene Expression Regulation Developmental Cell Differentiation DNA Articles Molecular biology Immunohistochemistry Sensory Systems Cell biology Rats Ophthalmology medicine.anatomical_structure Retinal ganglion cell nervous system Pregnancy Animal Female sense organs Stem cell Neural development |
| Zdroj: | Investigative ophthalmologyvisual science. 56(2) |
| ISSN: | 1552-5783 |
| Popis: | Purpose To understand how loss of citron kinase (CitK) affects retinal progenitor cells (RPCs) in the developing rat retina. Methods We compared knockout (KO) and wild-type (WT) retinae by immunohistochemistry. The TdT-mediated dUTP terminal nick-end labeling (TUNEL) assay was performed to determine cell death. Pulse-chase experiments using 5-ethynyl-2'-deoxyuridine (EdU) were carried out to interrogate RPC behavior and in turn neurogenesis. Results Reverse transcription-polymerase chain reaction analysis showed that CitK was expressed at embryonic day (E)12 and was turned off at approximately postnatal day (P)4. Immunohistochemistry showed CitK being localized as puncta at the apical end of the outer neuroblastic layer (ONBL). Analyses during embryonic development showed that the KO retina was of comparable size to that of WT until E13. However, by E14, there was a reduction in the number of S-phase RPCs with a concomitant increase in TUNEL+ cells in the KO retina. Moreover, early neurogenesis, as reflected by retinal ganglion cell production, was not affected. Postnatal analysis of the retina showed that ONBL in the KO retina was reduced to half the size of that in WT and showed further degeneration. Immunohistochemistry revealed absence of Islet1+ bipolar cells at P2, which was further confirmed by EdU pulse-chase experiments. The CitK KO retinae underwent complete degeneration by P14. Conclusions Our study showed that CitK is not required for a subset of RPCs before E14, but is necessary for RPC survival post E14. This in turn results in normal early embryonic neurogenesis, but severely compromised later embryonic and postnatal neurogenesis. |
| Databáze: | OpenAIRE |
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