Expression of the novel T cell activation molecule hpH4 in HIV-infected patients: correlation with disease status
| Autor: | Luigi Ortona, Donatella Buonfiglio, Carlo Rumi, Charles A. Janeway, Flavia Bottarel, Thea Bensi, Sergio Rutella, Umberto Dianzani, M. B. Lucia, Roberto Cauda |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Time Factors T cell Immunology Apoptosis HIV Infections CD38 CD8-Positive T-Lymphocytes HIV Envelope Protein gp120 Asymptomatic Virus NAD+ Nucleosidase Antigens CD Virology HIV Seropositivity medicine Humans IL-2 receptor ADP-ribosyl Cyclase Aged chemistry.chemical_classification Acquired Immunodeficiency Syndrome Membrane Glycoproteins biology Receptors Interleukin-2 T lymphocyte HLA-DR Antigens Middle Aged biology.organism_classification Flow Cytometry ADP-ribosyl Cyclase 1 Antigens Differentiation Infectious Diseases medicine.anatomical_structure chemistry Lentivirus HIV-1 Leukocytes Mononuclear medicine.symptom Glycoprotein |
| Zdroj: | AIDS research and human retroviruses. 16(6) |
| ISSN: | 0889-2229 |
| Popis: | We have described hpH4, a surface glycoprotein selectively expressed by activated T cells and mature thymocytes and displaying weak lateral association with CD4. The hpH4 expression pattern and biochemical features, together with analysis of its tryptic digest by peptide mass searching using MALDI-MS, suggested that it is a novel molecule. The aim of this work was to evaluate the peripheral blood T cell expression of hpH4 in HIV-infected patients and the interplay between HIV gp120 and hpH4, since both molecules interact with CD4. hpH4 expression during HIV-1 infection was evaluated by assessing 55 patients at various disease stages and following up 3 patients with primary infection and 3 patients with AIDS. hpH4 expression displayed a peak in the early phase of primary infection, dropped to control levels in the asymptomatic phase, and was newly expressed, at low levels, as AIDS developed. The expression kinetics were different than those shown by HLA-DR, CD25, and CD38. The most striking findings were the transient hpH4 expression peak displayed in the earliest stage, which was unique for hpH4. Incubation of T cells from normal donors with HIV gp120 induced transient hpH4 expression in resting CD4+ T cells and potentiated the hpH4 lateral association with CD4 in activated T cells. Moreover, hpH4 triggering inhibited gp120-induced death of CD4+ cells. Therefore, H4 expression may be a response to avoid apoptosis induced by HIV products. |
| Databáze: | OpenAIRE |
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